Oleanolic Acid Ameliorates Benign Prostatic Hyperplasia by Regulating PCNA-Dependent Cell Cycle Progression In Vivo and In Vitro.,Journal of Natural Products

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Oleanolic Acid Ameliorates Benign Prostatic Hyperplasia by Regulating PCNA-Dependent Cell Cycle Progression In Vivo and In Vitro.
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-03-19 , DOI: 10.1021/acs.jnatprod.9b01210
Se-Yun Cheon _{1,

2} , Bo-Ram Jin ₁ , Hyo-Jung Kim ₁ , Hyo-Jin An ₁

Affiliation

Oleanolic acid (OA) is a natural, biologically active pentacyclic triterpenoid found in Cornus officinalis. Although C. officinalis and OA have antiproliferative actions, the effects and mechanisms of OA in benign prostatic hyperplasia (BPH) are unclear. We examined the effect of OA in an animal model of testosterone-induced BPH. Male rats were injected with testosterone propionate with or without OA. The inhibitory effect of OA on BPH-1 cells was determined in vitro. Rats with BPH exhibited outstanding BPH symptoms, including prostatic enlargement, upregulated dihydrotestosterone and 5α-reductase 2 levels, and histological changes. Compared with the BPH group, the OA group showed fewer pathological alterations and regular androgen events. OA inhibited prostate cell proliferation by downregulating the expression of proliferating cell nuclear antigen (PCNA) and cell cycle markers in BPH-induced animals. This indicated that OA has superior therapeutic effect in the BPH animal model than finasteride. In vitro studies demonstrated upregulation of PCNA and cell cycle proteins, whereas OA clearly reduced this upregulation. Thus, OA may inhibit the development of BPH by targeting cell cycle progression markers. These suggest that OA is a potential agent for BPH treatment.

中文翻译：

齐墩果酸通过调节体内和体外PCNA依赖的细胞周期进程改善良性前列腺增生。

齐墩果酸（OA）是山茱off中发现的一种天然的，具有生物活性的五环三萜。尽管C. officinalis和OA具有抗增殖作用，但尚不清楚OA在良性前列腺增生（BPH）中的作用和机制。我们检查了OA在睾丸激素诱导的BPH动物模型中的作用。雄性大鼠注射有或没有OA的丙酸睾丸激素。在体外确定了OA对BPH-1细胞的抑制作用。BPH大鼠表现出突出的BPH症状，包括前列腺肿大，二氢睾丸激素和5α-还原酶2水平上调以及组织学改变。与BPH组相比，OA组的病理改变和雄激素事件较少。OA通过下调BPH诱导的动物中增殖细胞核抗原（PCNA）和细胞周期标记的表达来抑制前列腺细胞的增殖。这表明OA在BPH动物模型中比非那雄胺具有更好的治疗作用。体外研究表明PCNA和细胞周期蛋白上调，而OA明显减少了上调。因此，OA可以通过靶向细胞周期进程标志物来抑制BPH的发展。这些提示OA是BPH治疗的潜在药物。OA可通过靶向细胞周期进程标记物来抑制BPH的发展。这些提示OA是BPH治疗的潜在药物。OA可通过靶向细胞周期进程标记物来抑制BPH的发展。这些提示OA是BPH治疗的潜在药物。

更新日期：2020-03-19

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