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Ultrasensitive and Label-Free Detection of Cell Surface Glycan Using Nanochannel-Ionchannel Hybrid Coupled with Electrochemical Detector.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-26 , DOI: 10.1021/acs.analchem.0c00330 Fei-Fei Liu , Xiao-Ping Zhao , Xue-Wei Liao 1 , Wen-Yuan Liu , Yu-Ming Chen 2 , Chen Wang
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-26 , DOI: 10.1021/acs.analchem.0c00330 Fei-Fei Liu , Xiao-Ping Zhao , Xue-Wei Liao 1 , Wen-Yuan Liu , Yu-Ming Chen 2 , Chen Wang
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In this work, asymmetric nanochannel–ionchannel of porous anodic alumina (PAA) coupled with electrochemical detector was used for sensitive and label-free detection of cell surface glycan. The amplified ionic current caused by array nanochannels as well as the ionic current rectification (ICR) caused by asymmetric geometry endows PAA with sensitive ionic current response. Functionalized with the special molecular probe, the constructed nanofluidic device can be used for selective recognition and detection of glycan in a real-time and label-free format. In addition, due to the subnanosize of ionchannels, the probe immobilization and glycan recognition is carried out on the outer surface of PAA, avoiding the blockage of PAA nanochannel by samples, which promises the reproducibility and accuracy of the present method toward bioanalysis. Results show that the glycan concentration ranging from 10 fM to 10 nM can be successfully detected with a detection limit of ∼10 aM, which is substantially lower than most previous works. The designed strategy provides a valuable platform for sensitive and label-free detection of cell surface glycan, which acts as a promising candidate in pathological research and cancer diagnosis.
中文翻译：
纳米通道-离子通道杂化体与电化学检测器耦合，可对细胞表面聚糖进行超灵敏且无标签的检测。
在这项工作中，多孔阳极氧化铝（PAA）的不对称纳米通道-离子通道与电化学检测器结合用于细胞表面聚糖的灵敏且无标记的检测。阵列纳米通道引起的放大离子电流以及非对称几何结构引起的离子电流整流（ICR）使PAA具有灵敏的离子电流响应。借助特殊的分子探针功能化，构建的纳米流体装置可用于实时识别且无标签的聚糖选择性识别和检测。另外，由于离子通道的亚纳米尺寸，在PAA的外表面上进行了探针固定和聚糖识别，避免了样品对PAA纳米通道的阻塞，这保证了本方法对生物分析的可重复性和准确性。结果表明，可以成功检测到10 fM至10 nM的聚糖浓度，检测限为〜10 aM，这大大低于大多数以前的工作。该设计策略为细胞表面聚糖的敏感和无标记检测提供了一个有价值的平台，该平台可作为病理研究和癌症诊断中的有希望的候选者。
更新日期：2020-03-27
中文翻译：
纳米通道-离子通道杂化体与电化学检测器耦合，可对细胞表面聚糖进行超灵敏且无标签的检测。
在这项工作中，多孔阳极氧化铝（PAA）的不对称纳米通道-离子通道与电化学检测器结合用于细胞表面聚糖的灵敏且无标记的检测。阵列纳米通道引起的放大离子电流以及非对称几何结构引起的离子电流整流（ICR）使PAA具有灵敏的离子电流响应。借助特殊的分子探针功能化，构建的纳米流体装置可用于实时识别且无标签的聚糖选择性识别和检测。另外，由于离子通道的亚纳米尺寸，在PAA的外表面上进行了探针固定和聚糖识别，避免了样品对PAA纳米通道的阻塞，这保证了本方法对生物分析的可重复性和准确性。结果表明，可以成功检测到10 fM至10 nM的聚糖浓度，检测限为〜10 aM，这大大低于大多数以前的工作。该设计策略为细胞表面聚糖的敏感和无标记检测提供了一个有价值的平台，该平台可作为病理研究和癌症诊断中的有希望的候选者。
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