Organic–Inorganic Composite Nanorods as an Excellent Mimicking Peroxidases for Colorimetric Detection and Evaluation of Antioxidant,ACS Applied Bio Materials

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Organic–Inorganic Composite Nanorods as an Excellent Mimicking Peroxidases for Colorimetric Detection and Evaluation of Antioxidant
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-19 , DOI: 10.1021/acsabm.0c00198
Hao Liu ₁ , YanLei He ₁ , Ning Li ₁ , Zhenxue Liu ₁ , Xianxi Zhang ₂ , Xiao Zhang ₃ , Qingyun Liu ₁

Affiliation

N,N′-Dicarboxy methyl perylene diimide-coated CeO₂ nanorods (PDI/CeO₂ NR) were synthesized via an ultrasonic-assisted method. PDI/CeO₂ exhibits the superb mimic peroxidase functions confirmed by the catalyzed oxidation of TMB by hydrogen peroxide in less than 60 s along with color transformation from colorless to blue. The catalytic mechanism was confirmed to be an electronic transfer mechanism by fluorescence experiment. Thus, a visual colorimetric sensor was constructed for hydrogen peroxide detection with a low detection limit (LOD = 2.23 μM). Comparing the inhibition effects of l-cysteine (Cys), ascorbic acid (AA) and glutathione (GSH) on the catalytic oxidation of TMB, it can be found that they possessed different types of inhibition on the oxidation of TMB by H₂O₂ using PDI/CeO₂, and AA has better antioxidant effect, followed by Cys and GSH. On the basis of the excellent antioxidant effect of AA, a low-cost colorimetric sensor was also used to detect AA, and a lower LOD value (0.68 μM) was obtained in the linear range of 5.0–30 μM.

中文翻译：

有机-无机复合纳米棒作为一种出色的模拟过氧化物酶，用于比色检测和抗氧化剂评估

通过超声辅助方法合成了N,N'-二羧甲基苝二亚胺包覆的CeO ₂纳米棒(PDI/CeO _{2 NR)。}PDI/CeO ₂表现出极好的模拟过氧化物酶功能，这通过过氧化氢在不到 60 秒内催化氧化 TMB 以及颜色从无色变为蓝色得到证实。通过荧光实验证实催化机理为电子转移机理。因此，构建了一种用于过氧化氢检测的视觉比色传感器，具有低检测限（LOD = 2.23 μM）。比较l的抑制作用-半胱氨酸(Cys)、抗坏血酸(AA)和谷胱甘肽(GSH)对TMB的催化氧化作用，可以发现它们对使用PDI/CeO _2的H₂ O ₂氧化TMB具有不同类型的抑制作用， AA具有较好的抗氧化作用，其次是Cys和GSH。基于AA优异的抗氧化作用，还采用低成本的比色传感器对AA进行检测，在5.0~30 μM的线性范围内获得了较低的LOD值（0.68 μM）。

更新日期：2020-04-23

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