Graphene oxide (GO)‐based materials have been explored in biomedical applications as active engineered materials for diagnosis and therapy. Although a large number of studies have been carried out in the last years, aspects involving the orientation and elongation of cells on GO immobilized on polymeric nanofibers are still scarce. We investigated the interactions between skeletal muscle cells and GO immobilized on random and aligned electrospun nanofibers of poly(caprolactone) (PCL), a biocompatible and biodegradable polymer. Oxygen plasma was employed to modify the nanofiber polymer surface to enhance the interactions between the PCL fibers and GO. Scanning electron microscopy and confocal microscopy revealed the morphology and orientation of skeletal muscle cells (C2C12 cells) on random and aligned GO/PCL nanofibers. The approach employed here is useful to investigate the interaction of skeletal muscle cells with biocompatible polymer nanofibers modified with GO intended for cell scaffolds and tissue engineering.

中文翻译：

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含氧化石墨烯的无规和定向电纺纳米纤维的制备，用于骨骼肌细胞支架

基于氧化石墨烯（GO）的材料已在生物医学应用中得到探索，可以用作诊断和治疗的活性工程材料。尽管最近几年进行了大量研究，但是涉及固定在聚合物纳米纤维上的GO上细胞的取向和伸长的方面仍然很少。我们研究了骨骼肌细胞与固定在聚己内酯（PCL）（一种生物相容性和可生物降解的聚合物）的随机排列的电纺纳米纤维上的GO之间的相互作用。氧等离子体用于修饰纳米纤维聚合物表面，以增强PCL纤维和GO之间的相互作用。扫描电子显微镜和共聚焦显微镜显示了随机排列的GO / PCL纳米纤维上骨骼肌细胞（C2C12细胞）的形态和方向。