Flow Homogenization Enables a Massively Parallel Fluidic Design for High‐Throughput and Multiplexed Cell Isolation,Advanced Materials Technologies

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Flow Homogenization Enables a Massively Parallel Fluidic Design for High‐Throughput and Multiplexed Cell Isolation
Advanced Materials Technologies ( IF 6.4 ) Pub Date : 2020-03-18 , DOI: 10.1002/admt.201900960
Chinchun Ooi ₁ , Christopher M Earhart ₂ , Casey E Hughes ₃ , Jung-Rok Lee ₄ , Dawson J Wong ₅ , Robert J Wilson ₂ , Rajat Rohatgi ₆ , Shan X Wang ₇

Affiliation

Microfluidic devices are widely used for applications such as cell isolation. Currently, the most common method to improve throughput for microfluidic devices involves fabrication of multiple, identical channels in parallel. However, this “numbering up” only occurs in one dimension, thereby limiting gains in volumetric throughput. In contrast, macrofluidic devices permit high volumetric flow rates but lack the finer control of microfluidics. Here, it is demonstrated how a micropore array design enables flow homogenization across a magnetic cell capture device, thus creating a massively parallel series of microscale flow channels with consistent fluidic and magnetic properties, regardless of spatial location. This design enables scaling in two dimensions, allowing flow rates exceeding 100 mL h⁻¹ while maintaining >90% capture efficiencies of spiked lung cancer cells from blood in a simulated circulating tumor cell system. Additionally, this design facilitates modularity in operation, which is demonstrated by combining two different devices in tandem for multiplexed cell separation in a single pass with no additional cell losses from processing.

中文翻译：

流动均质化可实现大规模并行流体设计，以实现高通量和多重细胞分离

微流体装置广泛用于细胞分离等应用。目前，提高微流体装置通量的最常见方法涉及并行制造多个相同的通道。然而，这种“向上编号”仅发生在一维，从而限制了体积吞吐量的增益。相比之下，宏流体装置允许高体积流速，但缺乏微流体的更精细控制。在这里，展示了微孔阵列设计如何实现磁性细胞捕获装置上的流动均质化，从而创建一系列大规模并行的微尺度流动通道，无论空间位置如何，都具有一致的流体和磁性特性。该设计能够实现二维缩放，允许流速超过100 mL h ^-1 ，同时在模拟循环肿瘤细胞系统中保持>90%从血液中捕获肺癌细胞的效率。此外，这种设计促进了操作中的模块化，这一点通过串联组合两个不同的设备来实现单次多重细胞分离而得到证明，并且不会因处理而造成额外的细胞损失。

更新日期：2020-03-18

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