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Novel Chemo-Photothermal Therapy in Breast Cancer Using Methotrexate-Loaded Folic Acid Conjugated Au@SiO 2 Nanoparticles
Nanoscale Research Letters ( IF 5.5 ) Pub Date : 2020-03-19 , DOI: 10.1186/s11671-020-3295-1
Reza Agabeigi 1 , Seyed Hossein Rasta 2 , Mohammad Rahmati-Yamchi 1 , Roya Salehi 3 , Effat Alizadeh 1
Affiliation  

Abstract

Low level laser therapy (LLLT) is known as a safe type of phototherapy to target tumor tissue/cells. Besides, using targeted nanoparticles increases the successfulness of cancer therapy. This study was designed for investigating the combined effect of folate (FA)/Methotrexate (MTX) loaded silica coated gold (Au@SiO2) nanoparticles (NPs) and LLLT on the fight against breast cancer.

NPs were synthesized and characterized using FTIR, TEM and DLS-Zeta. The NPs had spherical morphology with mean diameter of around 25 nm and positive charge (+13.3 mV) while after conjugation with FA and MTX their net charge reduced to around -19.7 mV.

Our findings in cell uptake studies clearly showed enhanced cellular uptake of NPs after FA and MTX loaded NPs in both breast cancer cell lines especially on MDA-MB-231 due to high expression of folate receptors. The results indicated that LLLT had a proliferative effect on both breast cancer cell lines but in the presence of engineered breast cancer targeted nanoparticle, the efficacy of combination chemo-photothermal therapy was significantly increased using MTT assay (p<0.05), DAPI staining, and cell cycle findings. The highest apoptotic effect on breast cancer cell lines was observed in the cells exposed to a combination of MTX-FA loaded Au@SiO2 NP and LLLT proved by DAPI staining and cell cycle(by increasing the cell arrest in subG0/G1). Taken together a combination of chemotherapy and LLLT improves the potential of breast cancer therapy with minimum side effects.



中文翻译:


使用甲氨蝶呤负载叶酸共轭 Au@SiO 2 纳米颗粒治疗乳腺癌的新型化疗光热疗法


 抽象的


低强度激光疗法(LLLT)被认为是一种针对肿瘤组织/细胞的安全光疗类型。此外,使用靶向纳米颗粒可以提高癌症治疗的成功率。本研究旨在研究叶酸 (FA)/甲氨蝶呤 (MTX) 负载二氧化硅包覆金 (Au@SiO 2 ) 纳米颗粒 (NP) 和 LLLT 在对抗乳腺癌方面的综合作用。


使用 FTIR、TEM 和 DLS-Zeta 合成并表征了纳米颗粒。纳米粒子具有球形形态,平均直径约为 25 nm,带正电荷 (+13.3 mV),而与 FA 和 MTX 缀合后,其净电荷减少至约 -19.7 mV。


我们的细胞摄取研究结果清楚地表明,由于叶酸受体的高表达,在两种乳腺癌细胞系中加载 FA 和 MTX 的 NP 后,细胞对 NP 的摄取增强,尤其是在 MDA-MB-231 上。结果表明,LLLT 对两种乳腺癌细胞系均具有增殖作用,但在工程化乳腺癌靶向纳米颗粒存在的情况下,使用 MTT 测定,联合化疗光热疗法的疗效显着增加 (p<0 id=13>2 NP LLLT 通过 DAPI 染色和细胞周期(通过增加 subG0/G1 中的细胞停滞)证明,化疗和 LLLT 的组合可以提高乳腺癌治疗的潜力,同时副作用最小。

更新日期:2020-03-19
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