Cultivated sweet potato (Ipomoea batatas) is an important source of food for humans and domesticated animals. Here, we show that a B-box (BBX) family transcription factor, IbBBX24, is a regulator of the hormone jasmonic acid (JA) in I. batatas. When IbBBX24 was overexpressed or silenced, JA accumulation was increased or decreased, respectively. Consistently, RNA-seq analysis reveals that IbBBX24 modulates the genes involved in the JA pathway. IbBBX24 regulates JA responses by antagonizing a JA signaling repressor, IbJAZ10, which relieves IbJAZ10's repression of IbMYC2, a JA signaling activator. IbBBX24 binds to the IbJAZ10 and IbMYC2 promoters, and represses and activates their transcription, respectively. The interaction between IbBBX24 and IbJAZ10 interferes with IbJAZ10's repression of IbMYC2, thereby promoting the transcriptional activity of IbMYC2. Overexpression of IbBBX24 significantly increased Fusarium wilt disease, suggesting that JA responses play a crucial role in regulating Fusarium wilt resistance in sweet potato. Moreover, overexpression of IbBBX24 led to increased yield in sweet potato. Together, our findings indicate that IbBBX24 plays a pivotal role in regulating JA biosynthesis and signaling, increasing Fusarium wilt resistance and yield in sweet potato, thus providing a new candidate gene for developing elite crop varieties with enhanced pathogen resistance but without yield penalty.

栽培的甘薯（番薯）是人类和家畜的重要食物来源。在这里，我们显示了B-box（BBX）家族转录因子IbBBX24是batatas茉莉酸（JA）的调节剂。当IbBBX24过表达或沉默时，JA积累分别增加或减少。一致地，RNA-seq分析显示IbBBX24调控JA途径中涉及的基因。IbBBX24通过拮抗JA信号传导阻遏物IbJAZ10来调节JA响应，IbJAZ10减轻了IbJAZ10对JA信号传导激活剂IbMYC2的抑制。IbBBX24与IbJAZ10和IbMYC2启动子结合，并分别抑制和激活它们的转录。IbBBX24和IbJAZ10之间的相互作用会干扰IbJAZ10对IbMYC2的抑制，从而促进IbMYC2的转录活性。IbBBX24的过表达显着增加了枯萎病的发生，这表明JA反应在调节甘薯的枯萎病抗性中起关键作用。此外，IbBBX24的过表达导致甘薯产量增加。总之，我们的发现表明，IbBBX24在调节JA生物合成和信号传导，增加甘薯的枯萎病抗性和产量中起关键作用，从而为开发具有增强的病原体抗性但没有产量损失的优良农作物品种提供了新的候选基因。IbBBX24的过表达导致甘薯产量增加。总之，我们的发现表明，IbBBX24在调节JA生物合成和信号传导，增加甘薯的枯萎病抗性和产量中起关键作用，从而为开发具有增强的病原体抗性但没有产量损失的优良农作物品种提供了新的候选基因。IbBBX24的过表达导致甘薯产量增加。总之，我们的发现表明，IbBBX24在调节JA生物合成和信号传导，增加甘薯的枯萎病抗性和产量中起关键作用，从而为开发具有增强的病原体抗性但没有产量损失的优良农作物品种提供了新的候选基因。