Fagopyrum cymosum has been considered as a traditional medicinal plant that belongs to Fagopyrum, which has exhibited great pharmaceutical potential due to its abundant flavonoid accumulation. The hairy roots induced by Agrobacterium rhizogenes has been utilized to produce valuable specialized metabolites or reveals plant metabolic processes, whereas the underlying regulatory networks of flavonoid biosynthesis in hairy roots of F. cymosum remained unexplored. Here, the regulatory transcription factor TrMYB4 cloned from Trifolium repens with purple striped leaves was considered to investigate the mechanism of flavonoids biosynthesis in hairy roots of F. cymosum. Results showed that the expression of key genes involved in rutin biosynthesis pathway from TrMYB4 hairy roots were significantly up-regulated compared with non-transgenic hairy roots, while the content of total flavonoids and rutin in TrMYB4 hairy roots also increased consistently. It revealed the TrMYB4 transcription factor could regulate the rutin biosynthesis in F. cymosum. Meanwhile, our research provided a theoretical reference for the industrial production of rutin using F. cymosum hairy roots.

中文翻译：

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TrMYB4 转录因子调节 F. cymosum 毛根中芦丁的生物合成

Fagopyrum cymosum 一直被认为是属于Fagopyrum 的传统药用植物，由于其丰富的黄酮积累，显示出巨大的药用潜力。发根农杆菌诱导的毛状根已被用于产生有价值的专门代谢物或揭示植物代谢过程，而 F. cymosum 毛状根中类黄酮生物合成的潜在调控网络仍未被探索。在这里，从具有紫色条纹叶子的三叶草中克隆的调节转录因子 TrMYB4 被认为是研究 F. cymosum 毛状根中类黄酮生物合成的机制。结果表明，与非转基因发根相比，TrMYB4发根参与芦丁生物合成途径的关键基因表达显着上调，而TrMYB4毛状根中总黄酮和芦丁的含量也持续增加。它揭示了TrMYB4转录因子可以调节F. cymosum中的芦丁生物合成。同时，我们的研究为大花蒌毛状根工业化生产芦丁提供了理论参考。