Curcumin grafted hyaluronic acid modified pullulan polymers (Cur-HA-SPu) by chemical conjugation was designed and prepared, and its film may be used to accelerate wound healing and help to fight infection. The synthesis of Cur-HA-SPu polymer was characterized by FT-IR, ¹H NMR and DSC. Cur-HA-SPu film has a higher swelling ratio than that of HA-SPu film. Moreover, the good biocompatibility of Cur-HA-SPu polymer was confirmed by skin irritation, cytotoxicity and hemolysis tests. Compared to Cur, the MTT and proliferation test carried out in L929 cells revealed that Cur-HA-SPu polymer showed no cytotoxicity and enhanced cell proliferation. Cur-HA-SPu polymer exhibited a certain bactericidal activity against E.coli and S.aureus. Furthermore, the materials showed antioxidant activity when DPPH method determined. Wound healing study using wistar rat model demonstrated that Cur-HA-SPu film obtained better wound healing result than that of HA-SPu film or natural healing. The above results suggest that Cur-HA-SPu film is a promising and safety formulation for accelerating skin wound healing.

设计并制备了姜黄素通过化学缀合接枝的透明质酸修饰的支链淀粉聚合物（Cur-HA-SPu），其薄膜可用于加速伤口愈合并帮助抵抗感染。Cur-HA-SPu聚合物的合成通过FT-IR，¹ H NMR和DSC表征。Cur-HA-SPu膜具有比HA-SPu膜更高的溶胀率。此外，通过皮肤刺激，细胞毒性和溶血试验证实了Cur-HA-SPu聚合物具有良好的生物相容性。与Cur相比，在L929细胞中进行的MTT和增殖测试表明，Cur-HA-SPu聚合物没有细胞毒性，增强了细胞增殖。Cur-HA-SPu聚合物对大肠杆菌和金黄色葡萄球菌具有一定的杀菌活性。此外，当测定DPPH方法时，该材料显示出抗氧化活性。使用wistar大鼠模型进行的伤口愈合研究表明，Cur-HA-SPu膜比HA-SPu膜或自然愈合具有更好的伤口愈合效果。以上结果表明，Cur-HA-SPu膜是加速皮肤伤口愈合的有前途且安全的配方。