This study aimed to evaluate phytochemical and essential oil (EO) chemical composition of the lemon grass inoculated with Arbuscular mycorrhizal fungi (AMF) Rhizophagus clarus and Claroideoglomus etunicatum and with Azospirillum brasilense. The experimental design was completely randomized in a 2 × 3 factorial scheme: two levels of rhizobacterium (inoculated with A. brasilense and non-inoculated) and three levels of AMF (inoculated with R. clarus or C. etunicatum and non-inoculated). The experiment was carried out in a greenhouse with six treatments and 8 replications, totalizing 48 experimental units for four months. Total sugars (TS), reducing sugars (RS), phenolic content, flavonoids, antioxidant DPPH (2,2-diphenyl-1-picrylhydrazyl) activity and EO chemical composition were determined. Means were submitted to analysis of variance (ANOVA) and Duncan's test, also the data were submitted to analyses of hierarchical clustering and principal component. The RS increased with inoculation of C. etunicatum and/or A. brasilense and the TS increased with co-inoculation of R. clarus and A. brasilense. Total phenolic content in the lemon grass extract was 1600.1 μg g⁻¹ fresh mass when the plant was co-inoculated with C. etunicatum and A. brasilense, representing a significant increase of 112 % (p < 0.05) in comparison with the control. The levels of flavonoids increased with C. etunicatum inoculation and the antioxidant DPPH activity increased with A. brasilense inoculation. A possible increase in plants’ total metabolite rate is explained by symbiotic interaction between the AMF to improve root development. It was observed that the AMF C. etunicatum inoculation had better response to the host plant, and concluded that co-inoculation with C. etunicatum and A. brasiliense altered the EO content and chemical composition of lemon grass, being neral the major compound and the main precursor of total phenols.

这项研究旨在评估接种了丛枝菌根真菌（AMF）根瘤菌，克拉科球菌和巴西固氮菌的柠檬草的植物化学和精油（EO）化学成分。实验设计是随机化完全以2×3因子方案：根际细菌的两个级别（接种A. brasilense和未接种的）和三个级别的AMF（接种R. CLARUS或C. etunicatum和未接种）。该实验是在温室中进行的，该温室经过6次处理和8次重复实验，总共48个实验单位，为期4个月。测定总糖（TS），还原糖（RS），酚含量，类黄酮，抗氧化剂DPPH（2,2-二苯基-1-吡咯并肼基）活性和EO化学成分。将均值提交给方差分析（ANOVA）和Duncan检验，并将数据提交给层次聚类分析和主成分分析。该RS增加与接种C. etunicatum和/或A. brasilense和TS用的共接种增加R. CLARUS和A. brasilense。柠檬草提取物中的总酚含量为1600.1μgg ^-1将植物与C. etunicatum和A. brasilense一起接种时的新鲜质量，与对照相比显着增加了112％（p <0.05）。黄酮类物质具有提高水平C. etunicatum接种和抗氧化剂DPPH活性增加A. brasilense接种。AMF之间的共生相互作用改善了根的发育，从而解释了植物总代谢率可能增加的原因。观察到AMF C. etunicatum接种对宿主植物有更好的响应，并得出结论认为，与C. etunicatum和A. brasiliense一起接种 改变了柠檬草的EO含量和化学成分，成为总酚的主要化合物和主要前体。