Agriculture products spoilage caused by mold not only causes huge economic losses, but also poses a safety hazard to human health. Although essential oils (EOs) are widely used for agriculture products antisepsis, little is known about the synergistic antifungal mechanisms of their major components. In this paper, the synergistic mechanism of eugenol and citral (S_EC) on Penicillium roqueforti was studied. The results showed that S_EC caused irreversible damage to the cell membrane of P. roqueforti, resulting in significant changes in membrane protein conformation and fatty acid content, among which citral had a greater effect on fatty acid content than eugenol. Through the analysis of mitochondrial morphology and membrane potential, it was found that S_EC treatment resulted in serious collapse of mitochondria and significant increase of membrane potential. In this process, the contribution rate of eugenol was higher than that of citral. Further analysis of the tricarboxylic acid cycle (TCA) pathway showed that S_EC could inhibit the activity of key enzymes and the production of energy charge in the TCA pathway, resulting in significant down-regulation of gene expression of three key molecules (succinate dehydrogenase, isocitrate dehydrogenase and fumarate hydratase). In conclusion, these results indicated that S_EC could kill P. roqueforti through the tricarboxylic acid cycle.

霉菌引起的农产品变质不仅造成巨大的经济损失，而且对人体健康构成安全隐患。尽管香精油（EOs）广泛用于农产品防腐，但对其主要成分的协同抗真菌机理知之甚少。本文研究了丁子香酚和柠檬醛（S _EC）对罗氏青霉的协同作用机理。结果表明，S _EC对罗氏沼虾的细胞膜造成不可逆的损害。，导致膜蛋白构象和脂肪酸含量发生重大变化，其中柠檬醛比丁子香酚对脂肪酸含量的影响更大。通过对线粒体形态和膜电位的分析，发现S _EC处理导致线粒体严重塌陷，膜电位显着增加。在这个过程中，丁子香酚的贡献率高于柠檬醛。对三羧酸循环（TCA）途径的进一步分析表明，S _EC可能会抑制TCA途径中关键酶的活性和能量电荷的产生，从而导致三个关键分子（琥珀酸脱氢酶，异柠檬酸脱氢酶和富马酸水合酶）的基因表达显着下调。总之，这些结果表明，S _EC可以通过三羧酸循环杀死罗克福尔氏菌。