当前位置：
X-MOL 学术
›
Insect Biochem. Mol. Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Evaluation of five candidate receptors for three Bt toxins in the beet armyworm using CRISPR-mediated gene knockouts.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.ibmb.2020.103361 Jianlei Huang 1 , Yanjun Xu 1 , Yayun Zuo 1 , Yihua Yang 1 , Bruce E Tabashnik 2 , Yidong Wu 1
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.ibmb.2020.103361 Jianlei Huang 1 , Yanjun Xu 1 , Yayun Zuo 1 , Yihua Yang 1 , Bruce E Tabashnik 2 , Yidong Wu 1
Affiliation
|
Insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) can provide safe and effective control of some major pests, but evolution of resistance by pests diminishes these benefits. Better understanding of the genetics and mechanisms of resistance is urgently needed to improve methods for monitoring, managing, and countering pest resistance to Bt toxins. Here we used CRISPR-mediated knockouts to evaluate the role of five genes encoding candidate Bt toxin receptors in Spodoptera exigua (beet armyworm), a devastating pest of vegetable, field and flower crops. We compared susceptibility to Bt toxins Cry1Ac, Cry1Fa, and Cry1Ca between the parent susceptible strain and each of five strains homozygous for the knockout of one of the candidate genes (SeAPN1, SeCad1, SeABCC1, SeABCC2 or SeABCC3). The results from the 15 pairwise comparisons reveal that SeABCC2 has a major role and SeCad1 a minor role in mediating toxicity of Cry1Ac and Cry1Fa. SeABCC2 also has a minor role in toxicity of Cry1Ca. In addition, the results imply little or no role for the other three candidate receptors in toxicity of Cry1Ac or Cry1Fa; or for the four candidate receptors other than SeABCC2 in toxicity of Cry1Ca.
中文翻译：
使用CRISPR介导的基因敲除评估甜菜夜蛾中三种Bt毒素的五个候选受体。
来自苏云金芽孢杆菌（Btillus thuringiensis）（Bt）的杀虫蛋白可以提供对某些主要害虫的安全有效控制，但害虫产生抗药性会削弱这些益处。迫切需要更好地了解抗药性的遗传学和机制，以改善监测，管理和对抗害虫对Bt毒素的抗药性的方法。在这里，我们使用CRISPR介导的基因敲除来评估5个编码候选Bt毒素受体的基因在甜菜夜蛾（甜菜夜蛾）中的作用，甜菜夜蛾是一种破坏性的蔬菜，田地和花卉作物。我们比较了亲本易感菌株与纯合的五个候选菌株之一的Bt毒素Cry1Ac，Cry1Fa和Cry1Ca对敲除一种候选基因（SeAPN1，SeCad1，SeABCC1，SeABCC2或SeABCC3）的敏感性。15个成对比较的结果表明，SeABCC2在介导Cry1Ac和Cry1Fa的毒性中起主要作用，而SeCad1则起次要作用。SeABCC2在Cry1Ca的毒性中也起较小作用。另外，该结果暗示其他三种候选受体在Cry1Ac或Cry1Fa的毒性中作用很小或没有作用。或针对SeABCC2以外的四种候选受体的Cry1Ca毒性。
更新日期：2020-03-19
中文翻译：
使用CRISPR介导的基因敲除评估甜菜夜蛾中三种Bt毒素的五个候选受体。
来自苏云金芽孢杆菌（Btillus thuringiensis）（Bt）的杀虫蛋白可以提供对某些主要害虫的安全有效控制，但害虫产生抗药性会削弱这些益处。迫切需要更好地了解抗药性的遗传学和机制，以改善监测，管理和对抗害虫对Bt毒素的抗药性的方法。在这里，我们使用CRISPR介导的基因敲除来评估5个编码候选Bt毒素受体的基因在甜菜夜蛾（甜菜夜蛾）中的作用，甜菜夜蛾是一种破坏性的蔬菜，田地和花卉作物。我们比较了亲本易感菌株与纯合的五个候选菌株之一的Bt毒素Cry1Ac，Cry1Fa和Cry1Ca对敲除一种候选基因（SeAPN1，SeCad1，SeABCC1，SeABCC2或SeABCC3）的敏感性。15个成对比较的结果表明，SeABCC2在介导Cry1Ac和Cry1Fa的毒性中起主要作用，而SeCad1则起次要作用。SeABCC2在Cry1Ca的毒性中也起较小作用。另外，该结果暗示其他三种候选受体在Cry1Ac或Cry1Fa的毒性中作用很小或没有作用。或针对SeABCC2以外的四种候选受体的Cry1Ca毒性。
京公网安备 11010802027423号