In this study, crude oils extracted from spent coffee grounds (SCG) and olive pomace (OP) were used as raw-material to synthesize low-calorie triacylglycerols, either by acidolysis with capric acid, or by interesterification with ethyl caprate, in solvent-free media, catalyzed by sn-1,3 regioselective lipases. The Rhizopus oryzae lipase (ROL) was immobilized in magnetite nanoparticles (MNP–ROL) and tested as novel biocatalyst. MNP-ROL performance was compared with that of the commercial immobilized Thermomyces lanuginosus lipase (Lipozyme TL IM). For both oils, Lipozyme TL IM preferred interesterification over acidolysis. MNP-ROL catalyzed reactions were faster and acidolysis was preferred with yields of c.a. 50% new triacylglycerols after 3h acidolysis of OP or SCG oils. MNP-ROL was very stable following the Sadana deactivation model with half-lives of 163h and 220h when reused in batch acidolysis and interesterification of OP oil, respectively.

在这项研究中，从废咖啡渣（SCG）和橄榄果渣（OP）中提取的原油被用作合成低热量三酰基甘油的原料，方法是在溶剂中用癸酸进行酸解，或与癸酸乙酯进行酯交换。由sn- 1,3区域选择性脂肪酶催化的游离介质。的米根霉脂肪酶（ROL）固定在磁铁矿纳米颗粒（MNP-ROL）和作为生物催化剂的新测试。将MNP-ROL性能与商业化固定嗜热丝霉菌进行了比较脂肪酶（Lipozyme TL IM）。对于这两种油，Lipozyme TL IM均优先采用酯交换而不是酸解。MNP-ROL催化的反应更快，酸化是首选，在OP或SCG油酸化3h后，酸解的收率约为50％的新的三酰基甘油。MNP-ROL在遵循Sadana失活模型后非常稳定，分别用于间歇酸解和OP油酯交换时的半衰期分别为163h和220h。