Regarding the high requirement of cardiac and vascular implants in tissue engineering, a novel concept of surface chemistry strategy featuring multiple functions is proposed in this study, which provides glutathione peroxidase (GPx)-like catalytic activity and allows secondary reactions for grafting functional biomolecules. The suggested strategy is the fabrication of a metal-catechol-(amine) network (MCAN) containing copper ions with GPx-like activity, amine-bearing hexamethylenediamine (HD) and wet adhesive catechol dopamine (DA). With a simple one-step molecular/ion co-assembly, the developed copper-DA-HD (CuII-DA/HD) network can be used to catalyze the generation of therapeutic nitric oxide (NO) gas in a durable and dose-controllable manner. The primary amine groups in the CuII-DA/HD network facilitate the secondary immobilization of bivalirudin (BVLD) to further provide an antithrombotic activity as supplement to the functions of NO. The CuII-DA/HD + BVLD coating functionalized on cardiovascular stents successfully improved thromboresistance, anti-restenosis, and promotes re-endothelialization in vivo. With regard to the ease of operation and low cost, the synergetic modification using MCAN strategy is of great potential for developing multifunctional blood-contacting materials/devices.

中文翻译：

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用于表面协同催化修饰的金属-邻苯二酚-（胺）网络：产生治疗气体和生物分子接枝。

关于组织工程中对心脏和血管植入物的高要求，本研究提出了具有多种功能的表面化学策略的新概念，该概念提供了谷胱甘肽过氧化物酶（GPx）样的催化活性，并允许继发反应移植功能性生物分子。建议的策略是制造包含类似GPx活性的铜离子，含胺的六亚甲基二胺（HD）和湿式邻苯二酚多巴胺（DA）的金属邻苯二酚（胺）网络（MCAN）。通过简单的一步式分子/离子共组装，已开发的铜-DA-HD（CuII-DA / HD）网络可用于以持久且可控制的剂量催化一氧化氮（NO）气体的产生方式。CuII-DA / HD网络中的伯胺基团促进了比伐卢定（BVLD）的二级固定，从而进一步提供了抗血栓形成的活性，以补充NO的功能。在心血管支架上功能化的CuII-DA / HD + BVLD涂层成功改善了血栓抵抗性，抗再狭窄，并促进了体内的再内皮化。关于操作的简便性和低成本，使用MCAN策略的协同修饰对于开发多功能血液接触材料/装置具有巨大的潜力。