tiRNAs are small non-coding RNAs generated by angiogenin-mediated tRNA cleavage during cellular stress. Some tiRNAs were shown to be cytoprotective, while other reports indicate that the generation of tiRNAs is cytotoxic. We used rat model of focal cerebral ischemia-reperfusion (I/R) injury to study the generation and regulation of tiRNAs following in vivo I/R and the impact of neuroprotective therapy on their generation. tiRNAs were induced after I/R and Minocycline therapy reduced global tiRNA levels. Our results showed that tRNA cleavage is tRNA species specific, and neuroprotective treatment does not affect all tiRNA species. We also evaluated the temporal changes in several tRNA modifying enzymes and showed a correlation between their expression and tRNA cleavage. In conclusion, we show that tiRNAs can serve as biomarkers for stroke and stroke therapy, further adding them to the repertoire of tools that can be used to monitor and treat stroke.

中文翻译：

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应激诱导的tRNA减半（tiRNA）作为中风和中风治疗的生物标志物；临床前研究。

tiRNA是在细胞应激期间由血管生成素介导的tRNA裂解产生的小的非编码RNA。一些tiRNA被证明具有细胞保护作用，而其他报道则表明tiRNA的产生具有细胞毒性。我们使用大鼠局灶性脑缺血再灌注（I / R）损伤模型研究了体内I / R后tiRNA的产生和调控，以及神经保护疗法对其产生的影响。在I / R和Minocycline治疗降低整体tiRNA水平后诱导tiRNA。我们的结果表明，tRNA裂解是tRNA物种特异性的，并且神经保护性治疗并不影响所有tiRNA物种。我们还评估了几种tRNA修饰酶的时间变化，并显示了它们的表达与tRNA裂解之间的相关性。结论，