In this study we focused on gene expression and behavioral differences in mice with brain-specific Commd1 knockout. Commd1 is an imprinted gene with preferential maternal expression, residing within a larger genomic region previously found to affect sensorimotor gating. In this study, individuals harboring a conditional Commd1 mutant allele were bred with Syn1-Cre animals, paying special attention to the parent of origin of the Commd1 mutation. Analysis of mRNA levels of Commd1 and phenotypic tests, including the open field, sensorimotor gating, and the forced swim test, were conducted on offspring with either maternally or paternally derived Commd1 knockout. We found that measurable Commd1 mRNA knockout occurred only in the maternally derived line and affected stereotypy and depressive-like behavior without differences in total locomotion compared to controls. Interestingly, we found that maternal knockout animals exhibited decreased time swimming and increased time immobile when compared to maternal and paternal wild type, and paternal knockout animals. However, there were no differences in climbing behavior between genotypes. This study demonstrates an in vivo behavioral role for Commd1 for the first time and demonstrates the need for careful interpretation of experimental results involving Cre-based knockout systems.

中文翻译：

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神经元、父母特异性 Commd1 敲除对小鼠的行为影响。

在这项研究中，我们专注于大脑特异性 Commd1 敲除小鼠的基因表达和行为差异。Commd1 是一种具有优先母体表达的印记基因，位于先前发现影响感觉运动门控的较大基因组区域内。在这项研究中，携带条件 Commd1 突变等位基因的个体与 Syn1-Cre 动物繁殖，特别注意 Commd1 突变的起源父母。Commd1 的 mRNA 水平分析和表型测试，包括开放场、感觉运动门控和强迫游泳测试，对母系或父系衍生的 Commd1 敲除的后代进行。我们发现可测量的 Commd1 mRNA 敲除仅发生在母系衍生的系中，并影响刻板印象和抑郁样行为，与对照组相比，总体运动没有差异。有趣的是，我们发现与母系和父系野生型以及父系敲除动物相比，母系敲除动物的游泳时间减少，不动时间增加。然而，基因型之间的攀爬行为没有差异。这项研究首次证明了 Commd1 在体内的行为作用，并证明了需要仔细解释涉及基于 Cre 的敲除系统的实验结果。和父系淘汰动物。然而，基因型之间的攀爬行为没有差异。这项研究首次证明了 Commd1 在体内的行为作用，并证明了需要仔细解释涉及基于 Cre 的敲除系统的实验结果。和父系淘汰动物。然而，基因型之间的攀爬行为没有差异。这项研究首次证明了 Commd1 在体内的行为作用，并证明了需要仔细解释涉及基于 Cre 的敲除系统的实验结果。