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3D printed fluidic platform with in-situ covalently immobilized polymer monolithic column for automatic solid-phase extraction
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.033 Enrique Javier Carrasco-Correa 1 , David J Cocovi-Solberg 2 , José Manuel Herrero-Martínez 1 , Ernesto Francisco Simó-Alfonso 1 , Manuel Miró 3
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.033 Enrique Javier Carrasco-Correa 1 , David J Cocovi-Solberg 2 , José Manuel Herrero-Martínez 1 , Ernesto Francisco Simó-Alfonso 1 , Manuel Miró 3
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In this work, 3D stereolithographic printing is proposed for the first time for the fabrication of fluidic devices aimed at in-situ covalent immobilization of polymer monolithic columns. Integration in advanced flow injection systems capitalized upon programmable flow was realized for fully automatic solid-phase extraction (SPE) and clean-up procedures as a 'front-end' to on-line liquid chromatography. The as-fabricated 3D-printed extraction column devices were designed to tolerate the pressure drop of forward-flow fluidic systems when handling large sample volumes as demonstrated by the determination of anti-microbial agents, plastic additives and monomers as models of emerging contaminants (4-hydroxybenzoic acid, methylparaben, phenylparaben, bisphenol A and triclosan). Decoration of the monolithic phase with gold nanoparticles (AuNPs) was proven most appropriate for the enrichment of phenolic-type target compounds. In particular, the absolute recoveries for the tested analytes ranged from 73 to 92% both in water and saliva samples. The 3D printed composite monolith showed remarkable analytical features in terms of loading capacity (2 mg g-1), breakthrough volume (10 mL), satisfactory batch-to-batch reproducibility (<9% RSD), and easy on-line coupling of the SPE device to HPLC systems. The fully automatic 3D-printed SPE-HPLC hyphenated system was also exploited for the on-line extraction, matrix clean-up and determination of triclosan in 200 μL of real saliva samples.
中文翻译：
具有原位共价固定聚合物整体柱的 3D 打印流体平台，用于自动固相萃取
在这项工作中，首次提出3D立体光刻印刷用于制造旨在原位共价固定聚合物整体柱的流体装置。利用可编程流量实现了先进流动注射系统的集成，用于全自动固相萃取 (SPE) 和净化程序，作为在线液相色谱的“前端”。所制造的 3D 打印萃取柱装置旨在承受处理大样本量时正向流动流体系统的压降，如将抗菌剂、塑料添加剂和单体确定为新兴污染物模型所证明的那样 (4 -羟基苯甲酸、对羟基苯甲酸甲酯、对羟基苯甲酸苯酯、双酚A和三氯生）。事实证明，用金纳米颗粒 (AuNP) 修饰整体相最适合富集酚类目标化合物。特别是，水和唾液样品中测试分析物的绝对回收率为 73% 至 92%。3D 打印的复合整体材料在负载能力 (2 mg g-1)、突破体积 (10 mL)、令人满意的批次间重现性 (<9% RSD) 以及易于在线耦合等方面表现出显着的分析特征。 SPE 装置到 HPLC 系统。全自动 3D 打印 SPE-HPLC 联用系统还用于在线提取、基质净化和测定 200 μL 真实唾液样品中的三氯生。
更新日期：2020-05-01
中文翻译：
具有原位共价固定聚合物整体柱的 3D 打印流体平台，用于自动固相萃取
在这项工作中，首次提出3D立体光刻印刷用于制造旨在原位共价固定聚合物整体柱的流体装置。利用可编程流量实现了先进流动注射系统的集成，用于全自动固相萃取 (SPE) 和净化程序，作为在线液相色谱的“前端”。所制造的 3D 打印萃取柱装置旨在承受处理大样本量时正向流动流体系统的压降，如将抗菌剂、塑料添加剂和单体确定为新兴污染物模型所证明的那样 (4 -羟基苯甲酸、对羟基苯甲酸甲酯、对羟基苯甲酸苯酯、双酚A和三氯生）。事实证明，用金纳米颗粒 (AuNP) 修饰整体相最适合富集酚类目标化合物。特别是，水和唾液样品中测试分析物的绝对回收率为 73% 至 92%。3D 打印的复合整体材料在负载能力 (2 mg g-1)、突破体积 (10 mL)、令人满意的批次间重现性 (<9% RSD) 以及易于在线耦合等方面表现出显着的分析特征。 SPE 装置到 HPLC 系统。全自动 3D 打印 SPE-HPLC 联用系统还用于在线提取、基质净化和测定 200 μL 真实唾液样品中的三氯生。
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