2-(2H-Benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV 328) is an ultraviolet light (UV) absorber which prolongs the stability of plastics and other organic compounds towards UV radiation. Therefore, it is frequently used as a preserving additive and may result in an exposure of consumers. Thus, an analytical method using dispersive liquid-liquid microextraction and subsequent gas chromatography tandem mass spectrometry analysis with advanced electron ionization was developed for a human biomonitoring of UV 328 exposure, which enabled the determination of UV 328 and six of its metabolites in human urine. Sample preparation and derivatization were optimized. Baseline separation of the analytes was assured by the application of a suitable temperature program. The validation resulted in limits of detection of 0.1 µg/L for all analytes, variation coefficients from 1 to 12% for precision in series and from 5 to 12% for interday precision. Furthermore, relative recovery rates between 80 and 120% were determined. Moreover, the procedure was successfully applied to urine samples of a volunteer exposed to UV 328. The method showed excellent sensitivity, repeatability and robustness for all parameters and may be applicable for studies to elucidate the metabolism and kinetics of the investigated UV absorber and for monitoring of individuals with specific exposure to UV 328.

中文翻译：

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分散液-液微萃取和GC-MS / MS测定人尿中的紫外线吸收剂2-（2H-苯并三唑-2-基）-4,6-二叔戊基苯酚（UV 328）及其氧化代谢物。

2-（2H-苯并三唑-2-基）-4,6-二叔戊基苯酚（UV 328）是紫外线（UV）吸收剂，可延长塑料和其他有机化合物对UV辐射的稳定性。因此，它经常用作防腐添加剂，并可能导致消费者暴露。因此，开发了一种使用分散液-液微萃取和随后的气相色谱串联质谱分析以及先进电子电离的分析方法，用于人体对UV 328暴露的生物监测，从而能够测定人尿中的UV 328及其六种代谢物。优化了样品制备和衍生化。通过使用合适的温度程序，可以确保分析物的基线分离。通过验证，所有分析物的检出限为0.1 µg / L，连续系数的变化系数从1到12％，日间精度的变化系数从5到12％。此外，测定的相对回收率在80％至120％之间。此外，该程序已成功应用于暴露于UV 328的志愿者的尿液样品。该方法对所有参数均显示出极佳的灵敏度，可重复性和鲁棒性，可用于阐明被研究的紫外线吸收剂的代谢和动力学的研究以及监测特定暴露于紫外线328的人群。