Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.

中文翻译：

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臭豆触角中嗅觉相关基因的性别偏向基因表达（鳞翅目：龟科）

Conogethes pinicolalis（鳞翅目：Crombidae）类似于Conogethes punctiferalis（黄桃蛾），其寄主植物是裸子植物，尤其是马尾松。到目前为止，关于这种害虫的报道较少。在本研究中，我们首次对雄性和雌性C. pinicolalis的触角转录组进行了测序和表征。总共从松材线虫触角转录组中鉴定出26个气味结合蛋白（OBP）基因，19个化学感觉蛋白（CSP）基因，55个气味受体（OR）基因和20个离子受体（IR）基因，并注释了氨基酸序列C. punctiferalis的同系物。邻居相连的树表明，嗅觉相关基因的氨基酸序列与点状梭菌高度同源。此外，选择了参考基因，我们建议使用磷酸脱氢酶基因（GAPDH）或核糖体蛋白49基因（RP49）来验证幼虫发育阶段的目标基因表达，并验证成人组织的RP49或核糖体蛋白L13基因（RPL13）。我们的研究为C. pinicolalis和C. punctiferalis之间的分子水平表征提供了一个起点，这可能会为将来的有害生物管理研究提供支持。