Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.

中文翻译：

---

Conogethes pinicolalis（鳞翅目：Crambidae）触角中嗅觉相关基因的性别偏向基因表达


Conogethes pinicolalis（鳞翅目：Crambidae），与桃蛾Conogethes punctiferalis（黄桃蛾）相似，寄主植物为裸子植物，尤其是马尾松。迄今为止，有关该害虫的文献报道较少。在本研究中，我们首次对雄性和雌性 C. pinicolalis 的触角转录组进行了测序和表征。总共，从 C. pinicolalis 触角转录组中鉴定出 26 个气味结合蛋白（OBP）基因、19 个化学感应蛋白（CSP）基因、55 个气味受体（OR）基因和 20 个离子型受体（IR）基因，并注释了氨基酸序列C. punctiferalis 的同源物。邻接树表明嗅觉相关基因的氨基酸序列与桃花心木高度同源。此外，我们还选择了内参基因，推荐使用磷酸脱氢酶基因（GAPDH）或核糖体蛋白49基因（RP49）来验证幼虫发育阶段的目的基因表达，以及验证成体组织中的RP49或核糖体蛋白L13基因（RPL13）。我们的研究为 C. pinicolalis 和 C. punctiferalis 之间的分子水平表征提供了一个起点，这可能有助于未来的害虫管理研究。