Sheath blight (SB), caused by Rhizoctonia solani, is a common rice disease worldwide. Currently, rice cultivars with robust resistance to R. solani are still lacking. To provide theoretic basis for molecular breeding of R. solani-resistant rice cultivars, the changes of transcriptome profiles in response to R. solani infection were compared between a moderate resistant cultivar (Yanhui-888, YH) and a susceptible cultivar (Jingang-30, JG). In the present study, 3085 differentially express genes (DEGs) were detected between the infected leaves and the control in JG, with 2853 DEGs in YH. A total of 4091 unigenes were significantly upregulated in YH than in JG before infection, while 3192 were significantly upregulated after infection. Further analysis revealed that YH and JG showed similar molecular responses to R. solani infection, but the responses were earlier in JG than in YH. Expression levels of trans-cinnamate 4-monooxygenase (C4H), ethylene-insensitive protein 2 (EIN2), transcriptome factor WRKY33 and the KEGG pathway plant-pathogen interaction were significantly affected by R. solani infection. More importantly, these components were all over-represented in YH cultivar than in JG cultivar before and/or after infection. These genes possibly contribute to the higher resistance of YH to R. solani than JG and were potential target genes to molecularly breed R. solani-resistant rice cultivar.

中文翻译：

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水稻耐感品种和感病品种对茄枯萎病叶片转录的比较。

茄枯萎病是由世界范围内常见的水稻病引起的。目前，仍缺乏对茄红梭菌有很强抗性的水稻品种。为抗性水稻品种的分子育种提供理论依据，比较了中抗性品种Yanhui-888，YH和易感品种Jingang-30对抗性品种R.solani感染转录组谱的变化。 ，JG）。在本研究中，在JG的受感染叶片和对照之间检测到3085个差异表达基因（DEG），在YH中检测到2853个DEG。与感染前相比，YH中共有4091个单基因显着上调，而感染后共有3192个单基因显着上调。进一步的分析显示，YH和JG对sol。solani感染表现出相似的分子反应，但JG的回应要早于YH。solani感染显着影响反式肉桂酸4-单加氧酶（C4H），乙烯不敏感蛋白2（EIN2），转录组因子WRKY33和KEGG途径植物-病原体相互作用的表达水平。更重要的是，在感染之前和/或之后，这些成分在YH品种中比在JG品种中都过分表达。这些基因可能导致YH对So.solani的抗性高于JG，并且是分子上育种抗R. solani的水稻品种的潜在靶基因。在感染之前和/或之后，这些成分在YH品种中的表达均比在JG品种中的过量。这些基因可能导致YH对So.solani的抗性高于JG，并且是分子上育种抗R. solani的水稻品种的潜在靶基因。在感染之前和/或之后，这些成分在YH品种中的表达均比在JG品种中的过量。这些基因可能导致YH对So.solani的抗性高于JG，并且是分子上育种抗R. solani的水稻品种的潜在靶基因。