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Inexpensive and Versatile Paper-Based Platform for 3D Culture of Liver Cells and Related Bioassays
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-18 , DOI: 10.1021/acsabm.0c00237 Tarun Agarwal 1 , Pratik Biswas 1 , Sampriti Pal 1 , Tapas Kumar Maiti 1 , Suman Chakraborty 2 , Sudip Kumar Ghosh 1 , Riddhiman Dhar 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-18 , DOI: 10.1021/acsabm.0c00237 Tarun Agarwal 1 , Pratik Biswas 1 , Sampriti Pal 1 , Tapas Kumar Maiti 1 , Suman Chakraborty 2 , Sudip Kumar Ghosh 1 , Riddhiman Dhar 1
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The present study delineates the fabrication of paper-based devices for culturing liver cells and developing related bioassays. The devices were prepared by conventional lab-based LaserJet printing technology and employed for 3D cell culture. Our results demonstrated that the devices efficiently supported the growth of multiple cell types incuding HepG2, HUVEC, fibroblasts, and MSCs. We further showed that the device specifications (grade of paper or design parameters) greatly impacted the functional phenotype of the HepG2 cells. We also explored the application of the developed devices for routine cell culture, drug screening, coculture, and transwell migration assays. The cellular responses observed on the paper under different culture configurations were similar to those obtained in the case of tissue culture plate (TCP). Moreover, we showed that the paper-based devices were compatible with the immunocytochemistry and ELISA procedures (no indication of nonspecific matrix-antibody interaction). Considering the simplicity, experimental flexibility, cost-effectiveness, and multiplexibility of the paper-based liver models, it is deemed to be ideal for developing cell-based bioassays, especially in resource-limited settings.
中文翻译：
用于肝细胞 3D 培养和相关生物测定的廉价且多功能的纸质平台
本研究描述了用于培养肝细胞和开发相关生物测定的纸基设备的制造。这些设备是通过传统的基于实验室的 LaserJet 打印技术制备的，并用于 3D 细胞培养。我们的结果表明，这些设备有效地支持多种细胞类型的生长，包括 HepG2、HUVEC、成纤维细胞和 MSC。我们进一步表明，设备规格（纸张等级或设计参数）极大地影响了 HepG2 细胞的功能表型。我们还探索了开发的设备在常规细胞培养、药物筛选、共培养和跨孔迁移测定中的应用。在不同培养配置下在纸上观察到的细胞反应与在组织培养板 (TCP) 的情况下获得的细胞反应相似。而且，我们发现纸基设备与免疫细胞化学和 ELISA 程序兼容（没有迹象表明非特异性基质-抗体相互作用）。考虑到纸质肝脏模型的简单性、实验灵活性、成本效益和多重性，它被认为是开发基于细胞的生物测定的理想选择，尤其是在资源有限的环境中。
更新日期：2020-04-23
中文翻译：
用于肝细胞 3D 培养和相关生物测定的廉价且多功能的纸质平台
本研究描述了用于培养肝细胞和开发相关生物测定的纸基设备的制造。这些设备是通过传统的基于实验室的 LaserJet 打印技术制备的，并用于 3D 细胞培养。我们的结果表明，这些设备有效地支持多种细胞类型的生长，包括 HepG2、HUVEC、成纤维细胞和 MSC。我们进一步表明，设备规格（纸张等级或设计参数）极大地影响了 HepG2 细胞的功能表型。我们还探索了开发的设备在常规细胞培养、药物筛选、共培养和跨孔迁移测定中的应用。在不同培养配置下在纸上观察到的细胞反应与在组织培养板 (TCP) 的情况下获得的细胞反应相似。而且，我们发现纸基设备与免疫细胞化学和 ELISA 程序兼容（没有迹象表明非特异性基质-抗体相互作用）。考虑到纸质肝脏模型的简单性、实验灵活性、成本效益和多重性，它被认为是开发基于细胞的生物测定的理想选择，尤其是在资源有限的环境中。
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