Nicarbazin is an anticoccidial drug with a residue limit in animal husbandry. 4,4′-Dinitrocarbanilide (DNC) is the residue marker of nicarbazin. In this research, designed and based on new haptens, a sensitive and specific monoclonal antibody was produced against DNC. The IC₅₀ value of the prepared mAb 3A3 was 0.14 ng mL⁻¹ and the LOD was 0.02 ng mL⁻¹. The recovery rate of the chicken breast and liver samples were 93.50–115.60% and 99.60–108.00%, respectively. The results of the cross-reaction experiment show that it is highly specific for DNC. A colloidal gold immunochromatographic test strip was prepared for rapid detection of DNC in the chicken breast and liver samples. The visual limits (LODs) of DNC detection in the chicken breast and liver samples were 2.5 μg kg⁻¹ and 5 μg kg⁻¹, and the cut-offs were 10 μg kg⁻¹ and 10 μg kg⁻¹, respectively. Therefore, the preparation of colloidal gold immunochromatographic test strips can be meaningful for the on-site detection and rapid screening of numerous samples.

中文翻译：

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超灵敏免疫色谱带，可基于单克隆抗体快速筛查鸡胸肉和肝样品中的尼卡巴嗪标记

尼卡巴嗪是一种抗球虫药，在畜牧业中具有残留限量。4,4'-Dinitrocarbanilide（DNC）是尼卡巴嗪的残留标记。在这项基于新半抗原的设计和研究中，生产了针对DNC的敏感而特异性的单克隆抗体。制备的mAb 3A3的IC ₅₀值为0.14 ng mL ^-1，LOD为0.02 ng mL ^-1。鸡胸和肝样品的回收率分别为93.50–115.60％和99.60–108.00％。交叉反应实验的结果表明，它对DNC具有高度的特异性。制备了胶体金免疫色谱试纸，用于快速检测鸡胸和肝样品中的DNC。鸡胸肉和肝样品中DNC检测的视觉限度（LOD）为2.5μgkg ^-1和5μgkg ^-1，截断值分别为10μgkg ^-1和10μgkg ^-1。因此，胶体金免疫层析试纸的制备对大量样品的现场检测和快速筛选具有重要意义。