The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techniques. Fungus was screened for the production of secondary metabolites using a specific medium. The metabolites were extracted using a suitable solvent and each fraction was subsequently evaluated for their antioxidant, antimicrobial, antiproliferative and anti-aging properties. The ethanolic extract depicted the highest antioxidant activity with 83%, 79%, 80% and 74% as assessed by ferric reducing power, 2,2-diphenyl 1-picrylhydrazyl, 2,2′-azino-bis3-ethylbenzthiazoline-6-sulfonic and phosphomolybdenum assays, respectively. Similarly, ethanolic extracts depicted marked antimicrobial activity as compared with standard antibiotics and antifungal agents as well as demonstrated significant antiproliferative property against a panel of mammalian cancer cell lines. Furthermore, different fractions of the purified ethanolic extract obtained using adsorption column chromatography were evaluated for antiproliferative property and identification of an active metabolite in the purified fraction using gas chromatography–mass spectroscopy and nuclear magnetic resonance techniques yielded 3-methyl-4-oxo-pentanoic acid. Thus, the present study suggests that the active metabolite 3-methyl-4-oxo-pentanoic acid extracted from Talaromyces purpureogenus isolate-ABRF2 has a potential antiproliferative, anti-aging, and antimicrobial therapeutic properties that will be further evaluated using in vivo studies in future.

中文翻译：

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根际真菌Talaromyces purpuregenusus离体ABRF2的体内和体外抗氧化和抗增殖活性

本研究评估了从印度Achanakmar生物圈保护区分离的根际真菌的潜在生物活性。木耳，篮状菌属purpureogenus isolate- ABRF2利用形态学，生化和分子技术对Achanakmar生物圈土壤中的沙门氏菌进行了表征。使用特定培养基筛选真菌以产生次生代谢产物。使用合适的溶剂提取代谢物，然后评估每个馏分的抗氧化剂，抗微生物剂，抗增殖剂和抗衰老特性。乙醇提取物表现出最高的抗氧化活性，通过三价铁还原力，2,2-二苯基1-吡啶并肼基，2,2'-叠氮基-双3-乙基苯并噻唑啉-6-磺酸评估，其抗氧化剂活性最高，为83％，79％，80％和74％。和磷钼化验。同样，与标准抗生素和抗真菌剂相比，乙醇提取物具有显着的抗微生物活性，并且对一组哺乳动物癌细胞系具有显着的抗增殖特性。此外，使用气相色谱-质谱和核磁共振技术评估了通过吸附柱色谱法获得的纯化乙醇提取物的不同馏分的抗增殖性能，并鉴定了纯化馏分中的活性代谢产物，得到了3-甲基-4-氧代戊酸酸。因此，本研究表明，从中提取的活性代谢物3-甲基-4-氧代戊酸 使用气相色谱-质谱和核磁共振技术评估了使用吸附柱色谱法纯化的乙醇提取物中不同部分的抗增殖性能，并鉴定了纯化级分中的活性代谢产物，得到了3-甲基-4-氧代戊酸。因此，本研究表明，从中提取的活性代谢物3-甲基-4-氧代戊酸 使用气相色谱-质谱和核磁共振技术评估了使用吸附柱色谱法纯化的乙醇提取物中不同部分的抗增殖性能，并鉴定了纯化级分中的活性代谢产物，得到了3-甲基-4-氧代戊酸。因此，本研究表明，从中提取的活性代谢物3-甲基-4-氧代戊酸踝节菌属purpureogenus isolate- ABRF2具有潜在的抗增殖，将使用在未来体内研究进一步评价的抗老化和抗微生物的治疗性质。