Abstract Bone defects are a major threat to human health, and heterotopic bone provides advantages that other materials do not provide in the treatment of bone defects. MPEG-PCL microspheres loaded with bone morphogenetic protein-2 (BMP-2/MPEG-PCL-MS) are an emerging means of culturing heterotopic bone. However, the effect of such microspheres on the quality of heterotopic bone osteogenesis has rarely been studied. The performance of heterotopic bone as a bone repair material has also rarely been reported. To explore the effect of microspheres on heterotopic bone osteogenesis and to explore the performance of heterotopic bone in bone repair, we designed compression tests, nanoindentation tests, and a finite element (FE) simulation. The effects of microspheres on the quality of heterotopic bone osteogenesis and performance of heterotopic bone as a bone repair material were evaluated from the mechanical properties, material properties, and FE simulation. The results demonstrate that eight-week heterotopic bone using microspheres has suitable mechanical and material properties, performs well in bone repair FE simulation, and can meet the material requirements of bone repair. Hence, this heterotopic bone as bone repair material has promising potential for clinical applications, and provides a good strategy for combination between tissue engineering and pharmaceutical science.

中文翻译：

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骨形态发生蛋白2微球诱导的异位骨作为骨修复材料的性能

摘要 骨缺损是人类健康的主要威胁，异位骨在治疗骨缺损方面具有其他材料所不具备的优势。装载有骨形态发生蛋白-2 (BMP-2/MPEG-PCL-MS) 的 MPEG-PCL 微球是培养异位骨的新兴手段。然而，很少研究这种微球对异位骨成骨质量的影响。异位骨作为骨修复材料的性能也鲜有报道。为了探索微球对异位骨成骨的影响并探索异位骨在骨修复中的性能，我们设计了压缩测试、纳米压痕测试和有限元 (FE) 模拟。从力学性能、材料性能和有限元模拟评估微球对异位骨成骨质量和异位骨作为骨修复材料的性能的影响。结果表明，使用微球的八周异位骨具有合适的力学和材料性能，在骨修复有限元模拟中表现良好，能够满足骨修复的材料要求。因此，这种异位骨作为骨修复材料具有广阔的临床应用潜力，为组织工程与药物科学的结合提供了良好的策略。结果表明，使用微球的八周异位骨具有合适的力学和材料性能，在骨修复有限元模拟中表现良好，能够满足骨修复的材料要求。因此，这种异位骨作为骨修复材料具有广阔的临床应用潜力，为组织工程与药物科学的结合提供了良好的策略。结果表明，使用微球的八周异位骨具有合适的力学和材料性能，在骨修复有限元模拟中表现良好，能够满足骨修复的材料要求。因此，这种异位骨作为骨修复材料具有广阔的临床应用潜力，为组织工程与药物科学的结合提供了良好的策略。