Lactobacillus plantarum AR113, a strain isolated from Chinese traditional pickle, was evaluated for its survival capacity under different stress conditions such as low pH, high bile, high osmotic pressure and antibiotics, and its probiotic characteristics were measured subsequently. Furthermore, the mechanism of antioxidant in L. plantarum AR113 was also determined. It was showed that AR113 exhibited the shortest lag phase, and it could grow at initial pH 3.0 or 1.1% bile salt or 8.0% NaCl. AR113 also showed higher abilities of auto-aggregation and co-aggregation. Furthermore, AR113 was safety confidence such as antibiotic susceptibility. After analyzing the genes related to antioxidant, gene gshR and npx were found to up-regulated after treated with H₂O₂ (2 mM), which may provide the evidence of the antioxidant mechanism under H₂O₂ of AR113 at transcriptional level.