The state-of-the-art hernia meshes, used in hospitals for hernia repair, are predominantly polymeric textile-based constructs that present high mechanical strength, but lack antimicrobial properties. Consequently, preventing bacterial colonization of implanted prosthetic meshes is of major clinical relevance for patients undergoing hernia repair. In this study, the co-axial electrospinning technique was investigated for the development of a novel mechanically stable structure incorporating dual drug release antimicrobial action. Core/shell structured nanofibers were developed, consisting of Nylon-6 in the core, to provide the appropriate mechanical stability, and Chitosan/Polyethylene oxide in the shell to provide bacteriostatic action. The core/shell structure consisted of a binary antimicrobial system incorporating 5-chloro-8-quinolinol in the chitosan shell, with the sustained release of Poly(hexanide) from the Nylon-6 core of the fibers. Homogeneous nanofibers with a "beads-in-fiber" architecture were observed by TEM, and validated by FTIR and XPS. The composite nanofibrous meshes significantly advance the stress–strain responses in comparison to the counterpart single-polymer electrospun meshes. The antimicrobial effectiveness was evaluated in vitro against two of the most commonly occurring pathogenic bacteria; S. aureus and P. aeruginosa, in surgical site infections. This study illustrates how the tailoring of core/shell nanofibers can be of interest for the development of active antimicrobial surfaces.

中文翻译：

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尼龙6/壳聚糖核/壳抗菌纳米纤维用于预防网状手术部位感染

医院用于疝气修复的最先进的疝气网主要是基于聚合物织物的结构，具有高机械强度，但缺乏抗菌性能。因此，防止植入的假体网片的细菌定植对于接受疝修补术的患者具有重要的临床意义。在这项研究中，研究了同轴静电纺丝技术，以开发一种具有双重药物释放抗菌作用的新型机械稳定结构。开发了核/壳结构的纳米纤维，其核中含有尼龙6，以提供适当的机械稳定性，壳中含有壳聚糖/聚环氧乙烷以提供抑菌作用。核/壳结构由在壳聚糖壳中掺入 5-氯-8-羟基喹啉的二元抗菌系统组成，并从纤维的 Nylon-6 核中持续释放聚己缩醛。通过 TEM 观察具有“纤维中珠”结构的均质纳米纤维，并通过 FTIR 和 XPS 进行验证。与对应的单一聚合物电纺网相比，复合纳米纤维网显着提高了应力应变响应。针对两种最常见的致病菌在体外评估了抗菌效果；金黄色葡萄球菌和铜绿假单胞菌，用于手术部位感染。这项研究说明了核/壳纳米纤维的定制如何对活性抗菌表面的开发产生兴趣。