Two Elongases, Elovl4 and Elovl6, Fulfill the Elongation Routes of the LC-PUFA Biosynthesis Pathway in the Orange Mud Crab (Scylla olivacea),Journal of Agricultural and Food Chemistry

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Two Elongases, Elovl4 and Elovl6, Fulfill the Elongation Routes of the LC-PUFA Biosynthesis Pathway in the Orange Mud Crab (Scylla olivacea)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-03-30 , DOI: 10.1021/acs.jafc.9b06692
Seng Yeat Ting ₁ , M. Janaranjani ₁ , P. Merosha ₂ , Ka-Kei Sam ₁ , Swe Cheng Wong ₃ , Pei-Tian Goh ₂ , Min-Qian Mah ₂ , Meng-Kiat Kuah ₁ , Alexander Chong Shu-Chien _{1,

2}

Affiliation

While the capacity for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis has been elucidated in vertebrates and several invertebrate phyla, the comparative knowledge in crustaceans remains vague. A key obstacle in mapping the full spectrum of LC-PUFA biosynthesis in crustacean is the limited evidence of the functional activities of enzymes involved in desaturation or elongation of polyunsaturated fatty acid substrates. In this present study, we report on the cloning and functional characterization of two Elovl elongases from the orange mud crab, Scylla olivacea. Sequence and phylogenetic analysis suggest these two Elovl as putative Elovl4 and Elovl6, respectively. Using the recombinant expression system in Saccharomyces cerevisiae, we demonstrate the elongation capacity for C18-C22 PUFA substrates in the S. olivacea Elovl4. The S. olivacea Elovl6 elongated saturated fatty acids, monounsaturated fatty acids, and interestingly, C18-C20 PUFA. Taken together, both Elovl fulfill the elongation steps required for conversion of C18 PUFA to their respective LC-PUFA products. Elovl4 is expressed mainly in the hepatopancreas and gill tissues, while Elovl6 is predominant in digestive tissues. The mRNA expression of both enzymes was higher in mud crabs fed with vegetable oil-based diets. Tissue fatty acid composition also showed the existence of LC-PUFA biosynthesis intermediate products in tissues expressing these two elongases. In summary, we report here two novel Elovl with PUFA elongating activities in a marine brachyuran. This will contribute significantly to the understanding of the LC-PUFA biosynthesis pathway in crustaceans and advance the development of aquafeed for intensive farming of the mud crab.

中文翻译：

两种延伸酶Elovl4和Elovl6满足了橙泥蟹（Scyla olivacea）中LC-PUFA生物合成途径的延伸途径

尽管在脊椎动物和一些无脊椎动物门中已经阐明了长链多不饱和脂肪酸（LC-PUFA）生物合成的能力，但在甲壳类动物中的比较知识仍然不明确。绘制甲壳动物中LC-PUFA生物合成全谱图的主要障碍是参与多不饱和脂肪酸底物去饱和或延伸的酶功能活性的有限证据。在本研究中，我们报道了橙泥蟹Scylla olivacea中两种Elovl延伸酶的克隆和功能表征。序列分析和系统发育分析表明这两个Elovl分别为推定的Elov14和Elov16。在酿酒酵母中使用重组表达系统，我们证明了S. olivacea Elovl4中C18-C22 PUFA底物的延伸能力。的S.蛤ELOVL6细长饱和脂肪酸，单不饱和脂肪酸，和有趣的是，C18-C20 PUFA。两者合计，两个Elovl均完成了将C18 PUFA转化为其各自的LC-PUFA产品所需的延伸步骤。Elovl4主要在肝胰腺和g组织中表达，而Elovl6主要在消化组织中表达。该基因在以植物油为基础的日粮中喂养的蟹蟹中，两种酶的表达都较高。组织脂肪酸组成还表明在表达这两种延长酶的组织中存在LC-PUFA生物合成中间产物。总而言之，我们在这里报告了两种新颖的Elovl，具有在海洋短臂猿中延长PUFA的活性。这将大大有助于了解甲壳动物中LC-PUFA的生物合成途径，并促进用于集约化养殖蟹的水产饲料的发展。

更新日期：2020-03-30

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