Monacolin K (MK) is a secondary metabolite synthesized by polyketide synthases of Monascus spp. In this study, the combined supplementation of three medicines, including Citri Reticulatae Pericarpium (CRP), Fructus crataegi (FC), and Radix Angelicae Dahuricae (RAD), were mixed with nonglutinous rice and were optimized by response surface methodology to enhance the production of MK in fermented red mold rice (RMR). Under the optimum condition, MK production achieved 3.60 mg/g, which was 41.18% higher than RMR without medicines. The improved MK production was mainly caused by the up‐regulated transcription level of mokA, mokB, mokF, mokH, mokI, and mplaeA. Meanwhile, the inhibitory effect of Poria cocos (PC) on MK production (only 0.436 mg/g) was caused by significantly down‐regulated transcription of six tested genes. Therefore, this study is beneficial for better understanding of the possible mechanism of enhanced MK production by optimization of fermentation conditions.

中文翻译：

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中药对红霉大米发酵中红曲霉莫纳可林钾的产生及相关基因转录的影响。

莫纳可林K（MK）是由红曲菌的聚酮化合物合酶合成的次级代谢产物。在这项研究中，将网状柠檬皮（CRP），山茱F（FC）和当归大头参（RAD）三种药物的组合补充剂与非糯米混合，并通过响应面法进行了优化，以提高产量。发酵红霉米（RMR）中的MK。在最佳条件下，MK产量达到3.60 mg / g，比不使用药物的RMR高出41.18％。MK产生的改善主要是由于mokA，mokB，mokF，mokH，mokI和mplaeA。同时，Por（PC）对MK产生的抑制作用（仅0.436 mg / g）是由于六个测试基因的转录明显下调所致。因此，这项研究有利于更好地了解通过优化发酵条件来提高MK产量的可能机理。