UCH‐L3 promotes non‐small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis,Biotechnology and Applied Biochemistry

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UCH‐L3 promotes non‐small cell lung cancer proliferation via accelerating cell cycle and inhibiting cell apoptosis
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-03-17 , DOI: 10.1002/bab.1909
Guoping Li ₁ , Xiaosheng Jin ₁ , Jisheng Zheng ₁ , Na Jiang ₁ , Wang Shi ₂

Affiliation

Ubiquitin C‐terminal hydrolase‐L3 (UCH‐L3) is a deubiquitinase that has a crucial role in oncogenesis. This study was aimed to explore the biological function of UCH‐L3 in non‐small cell lung cancer (NSCLC). Bioinformatics analysis was used to detect UCH‐L3 expression in NSCLC tissues and normal lung tissues, and to analyze the relationship between UCH‐L3 expression and survival of patients. qRT‐PCR and western blotting assays were used to detect UCH‐L3 expression in NSCLC tumor tissues and adjacent normal tissues. CCK‐8 assay was performed to examine the effect of UCH‐L3 on NSCLC cell proliferation. Flow cytometry assay was conducted to examine the effect of UCH‐L3 on NSCLC cell cycle and apoptosis. The expression of UCH‐L3 in NSCLC tissues was markedly higher than in normal lung tissues, and high expression of UCH‐L3 was positively associated with the poor survival of patients. UCH‐L3 knockdown significantly inhibited the proliferation of NSCLC cells, whereas UCH‐L3 overexpression had the opposite effect. Moreover, UCH‐L3 promoted NSCLC cells proliferation via accelerating cell cycle and inhibiting cell apoptosis. UCH‐L3 is upregulated in NSCLC and positively associated with the poor survival, and its expression contributes to NSCLC cell proliferation by accelerating cell cycle and inhibiting cell apoptosis.

中文翻译：

UCH‐L3通过加速细胞周期和抑制细胞凋亡来促进非小细胞肺癌的增殖

泛素C末端水解酶L3（UCH-L3）是一种去泛素酶，在肿瘤发生中起关键作用。这项研究旨在探讨UCH-L3在非小细胞肺癌（NSCLC）中的生物学功能。生物信息学分析用于检测NSCLC组织和正常肺组织中UCH-L3的表达，并分析UCH-L3的表达与患者生存之间的关系。使用qRT-PCR和Western印迹法检测UCL-L3在NSCLC肿瘤组织和邻近正常组织中的表达。进行CCK-8分析以检查UCH-L3对NSCLC细胞增殖的影响。进行了流式细胞仪分析以检查UCH-L3对NSCLC细胞周期和凋亡的影响。非小细胞肺癌组织中UCH-L3的表达明显高于正常肺组织，UCH-L3的高表达与患者存活率低呈正相关。UCH‐L3的抑制显着抑制了NSCLC细胞的增殖，而UCH‐L3的过表达则具有相反的作用。此外，UCH-L3通过加速细胞周期和抑制细胞凋亡来促进NSCLC细胞增殖。UCH-L3在NSCLC中上调，并与不良生存呈正相关，其表达通过加速细胞周期和抑制细胞凋亡而促进NSCLC细胞增殖。

更新日期：2020-03-17

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