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Surface Display of Complex Enzymes by in Situ SpyCatcher-SpyTag Interaction.
ChemBioChem ( IF 3.2 ) Pub Date : 2020-03-17 , DOI: 10.1002/cbic.202000102
Sabrina Gallus 1 , Theo Peschke 1, 2 , Malte Paulsen 3 , Teresa Burgahn 1 , Christof M Niemeyer 1 , Kersten S Rabe 1
Affiliation  

Room for independent folding . A surface display method employs the SpyCatcher‐SpyTag system for post‐translational in situ coupling of independently expressed and properly folded passenger enzymes and membrane anchors. The efficient surface display of delicate and complex enzymes expands the toolbox for the fabrication of productive whole‐cell biocatalysts.
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中文翻译:

通过原位 SpyCatcher-SpyTag 相互作用进行复杂酶的表面展示。

独立折叠室。表面展示方法采用 SpyCatcher-SpyTag 系统进行独立表达和正确折叠的过客酶和膜锚的翻译后原位偶联。精致而复杂的酶的有效表面展示扩展了生产高效全细胞生物催化剂的工具箱。
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更新日期:2020-03-17
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