A Proteolytic Site-Directed Affinity Label to Inhibit the Human ATP-Dependent Protease Caseinolytic Complex XP.,ChemBioChem

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A Proteolytic Site-Directed Affinity Label to Inhibit the Human ATP-Dependent Protease Caseinolytic Complex XP.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-03-16 , DOI: 10.1002/cbic.202000031
Zhou Sha ₁ , Sujatha Chilakala _{2,

3} , George Crabill _{1,

4} , Iteen Cheng _{1,

5} , Yan Xu ₂ , Jennifer Fishovitz ₆ , Irene Lee ₁

Affiliation

Enzyme‐specific inhibitors of the ATP‐dependent Lon and ClpXP proteases are necessary to distinguish the activity of these two similar enzymes in a protein mixture. We present here the design, synthesis, and characterization of a ClpXP‐specific inhibitor that allows mitochondrial Lon to be monitored in HeLa cell lysate.

中文翻译：

一个蛋白水解定点亲和标签，以抑制人类ATP依赖性蛋白酶酪蛋白水解复合物XP。

区分ATP的Lon和ClpXP蛋白酶的酶特异性抑制剂对于区分蛋白质混合物中这两种相似酶的活性是必需的。我们在此介绍ClpXP特异性抑制剂的设计，合成和表征，该抑制剂可在HeLa细胞裂解物中监测线粒体Lon。

更新日期：2020-03-16

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