As one of the reactive oxygen species (ROS), the concentration of hypochlorite (HClO/ClO⁻) in the environment and in vivo is associated with many diseases and is also quite significant for human health. In this paper, a novel ratiometric fluorescent probe CSN based on thieno[3,2-c]coumarin has been constructed for the detection of hypochlorite. This probe exhibited high selectivity and sensitivity towards HClO/ClO⁻, and showed a large emission shift (170 nm) and a 338-fold enhancement in the emission ratio (I₄₇₀/I₆₄₀). Moreover, investigations for sensing hypochlorite in real water samples and bioimaging in living cells were carried out, and the test results have confirmed the high potential of probe CSN in analytical and bioanalytical applications.

中文翻译：

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基于硫杂香豆素的新型比例荧光探针及其在实际水样和体内选择性检测次氯酸盐的应用

作为反应性氧物质（ROS）的一个，次氯酸盐的浓度（高氯酸/ CLO ^- ）在环境和体内与许多疾病相关联，并且也对人类健康相当显著。本文构建了一种基于硫代[3,2- c ]香豆素的新型比例荧光探针CSN，用于检测次氯酸盐。该探针显示出高的选择性和灵敏度的HClO朝/ CLO ^{- ，}并且显示在发光比率（大发射移（170纳米）和338倍的增强我₄₇₀ /我₆₄₀）。此外，进行了感测真实水样中次氯酸盐和活细胞生物成像的研究，测试结果证实了探针CSN在分析和生物分析应用中的巨大潜力。