Selective detection of sulfide in human lung cancer cells with a blue-fluorescent "ON-OFF-ON" benzimidazole-based chemosensor ensemble.,Dalton Transactions

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Selective detection of sulfide in human lung cancer cells with a blue-fluorescent "ON-OFF-ON" benzimidazole-based chemosensor ensemble.
Dalton Transactions ( IF 3.5 ) Pub Date : 2020-04-08 , DOI: 10.1039/d0dt00031k
Chak-Shing Kwan ₁ , Tao Wang , Sing-Ming Chan , Zongwei Cai , Ken Cham-Fai Leung

Affiliation

A completely water-soluble, high quantum yield blue-fluorescent benzimidazole derivative (AQ), containing a rigid benzimidazole-thiophene structure, was synthesized. Among 21 metal ions, the fluorescence of AQ was selectively turned off by Cu2+ to form an AQ-Cu2+ ensemble. Thereafter, the fluorescence of the AQ-Cu2+ ensemble was turned on by sulfide (S2-) with high selectivity and sensitivity in pure water solution. In comparison with AQ-Ag+ and AQ-Hg2+ ensembles, AQ-Cu2+ was the only ensemble that was capable of detecting a sulfide anion. Also, the fluorescence intensity of AQ was linearly proportional to the concentration of Cu2+ and S2-. Both Cu2+ and S2- were detected within a minute in vitro. Moreover, AQ worked best in the pH range of 5-10 and had a limit of detection of 50 nM and 354 nM for Cu2+ and S2- respectively. It was employed for the detection of sulfide in human lung cancer A549 cells with low cytotoxicity.

中文翻译：

使用基于苯并咪唑的蓝色荧光“开-关-开”化学传感器组合选择性检测人肺癌细胞中的硫化物。

合成了具有刚性苯并咪唑-噻吩结构的完全水溶性，高量子产率的蓝色荧光苯并咪唑衍生物（AQ）。在21种金属离子中，AQ的荧光被Cu2 +选择性关闭，形成AQ-Cu2 +系综。此后，在纯水溶液中，硫化物（S2-）以高选择性和高灵敏度打开AQ-Cu2 +系团的荧光。与AQ-Ag +和AQ-Hg2 +乐团相比，AQ-Cu2 +是唯一能够检测硫化物阴离子的乐团。另外，AQ的荧光强度与Cu2 +和S2-的浓度成线性比例。在一分钟内在体外检测到Cu2 +和S2-。此外，AQ在5-10的pH范围内效果最好，对Cu2 +和S2-的检出限分别为50 nM和354 nM。

更新日期：2020-03-17

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