Metabolic mapping with plasmonic nanoparticle assemblies,Analyst

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Metabolic mapping with plasmonic nanoparticle assemblies
Analyst ( IF 3.6 ) Pub Date : 2020/03/17 , DOI: 10.1039/c9an02262g
Nguyen H. Le _{1,

2,

3,

4} , Gang Ye _{2,

3,

4,

5} , Chun Peng _{2,

3,

4,

5,

6} , Jennifer I. L. Chen _{1,

2,

3,

4}

Affiliation

A rapid and simple methodology for the biomolecular analysis of single cells and microenvironments via a stick-and-peel plasmonic sensing platform is reported. Substrate-bound assemblies of plasmonic gold nanoparticles linked by reconfigurable oligonucleotides undergo disassembly upon target binding. Changes in the light scattering intensity of thousands of discrete nanoparticle assemblies are extrapolated concomitantly to yield the mapping of local target concentrations. The methodology is completely free of labelling, purification and separation steps. We quantified the intracellular ATP levels for two ovarian cancer cell lines to elucidate the differences and cellular distribution, and demonstrated the potential of the stick-and-peel platform for mapping the microenvironment of a 2D heterogeneous surface. The portable and economical analytical platform may broaden the affordability and applicability of single-cell based analyses and enable new opportunities in clinical care such as on-site molecular pathology.

中文翻译：

等离子纳米粒子组件的代谢图谱

一种快速简单的方法，可通过以下方法对单个细胞和微环境进行生物分子分析报道了一种粘皮等离子传感平台。通过可重组寡核苷酸连接的等离激元金纳米颗粒的基质结合组装体在靶标结合后发生分解。随之推断出数千个离散纳米粒子组件的光散射强度变化，以绘制局部目标浓度的图。该方法完全没有标记，纯化和分离步骤。我们量化了两个卵巢癌细胞系的细胞内ATP水平，以阐明差异和细胞分布，并证明了粘滞和剥离平台可用于绘制二维异质表面的微环境。

更新日期：2020-03-31

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