As the physical barrier between the cell and the outside environment, the plasma membrane is well-positioned to be the first responder to stress. The membrane is also highly vulnerable to many types of perturbation, including heat, force, osmotic pressure, lipid shortage, and starvation. To determine whether the structural changes in the plasma membrane of Saccharomyces cerevisiae brought about by nutrient stress can be communicated to regulatory networks within the cell, we identified proteins that interact with stress granules (SGs), subcellular structures composed of proteins, and nontranslated RNAs that form when cells are stressed. We found that SG proteins interacted with components of eisosomes, which are subcortical membrane structures with a distinct lipid and protein composition. In response to starvation-triggered phosphorylation of eisosome proteins, eisosomes clustered and recruited SG components, including active Pkc1. The absence of eisosomes impaired SG formation, resulting in delayed recovery from nutrient deprivation. Thus, eisosome clustering is an example of interdomain communication in response to stress and identifies a previously unknown mechanism of SG regulation.

作为细胞和外部环境之间的物理屏障，质膜处于有利地位，可以成为压力的第一反应者。膜也很容易受到许多类型的干扰，包括热、力、渗透压、脂质短缺和饥饿。确定酿酒酵母质膜是否发生结构变化由营养应激引起的可以传达到细胞内的调节网络，我们确定了与应激颗粒 (SG)、由蛋白质组成的亚细胞结构以及细胞受到应激时形成的非翻译 RNA 相互作用的蛋白质。我们发现 SG 蛋白与 eisosomes 的成分相互作用，eisosomes 是具有独特脂质和蛋白质组成的皮层下膜结构。为了响应饥饿触发的 eisosome 蛋白磷酸化，eisosomes 聚集并招募 SG 成分，包括活性 Pkc1。缺乏 eisosomes 会损害 SG 的形成，导致从营养缺乏中恢复延迟。因此，eisosome 聚类是响应压力的域间通信的一个例子，并确定了以前未知的 SG 调节机制。