In this study, rutin was detected as a main component after the phytochemical screening of flavonoids from an ethanolic extract of banana leaves (Musa balbisiana) using thin layer chromatography plates (TLC). The rutin from the crude extract was separated using a series of solvent partition separations and was purified by using Sephadex™ column chromatography and semi-preparative reverse phase-high performance liquid chromatography (RP-HPLC). The compounds were characterized and quantified using NMR, Mass Spectroscopy (MS) and HPLC. A crude ethanolic extract of 124 g, containing 5.3 % of rutin, was obtained from 1 kg of dried banana leaf powder. After a series of solvent partition separations, the product from partitions using hexane/dichloromethane and dichloromethane/water in sequence contained a rutin content of 40.6 %. Purification using a Sephadex column yielded fractions with purity of rutin up to 74–84 % and further purification using semi-preparative RP-HPLC produced a final product with a yield of rutin of 32.4 mg/g of the crude extracts and a purity of 98.4 %. The results revealed that banana leaves (M. balbisiana), a food industry by-product and agricultural waste, has the potential for use as an inexpensive and new source of rutin.

在这项研究中，芦丁被检测为从香蕉叶的乙醇提取物的类黄酮的植物化学物质的筛选后的主成分（野蕉）使用薄层色谱板（TLC）。使用一系列溶剂分配分离法分离粗提物中的芦丁，并使用Sephadex™柱色谱和半制备型反相高效液相色谱（RP-HPLC）进行纯化。使用NMR，质谱（MS）和HPLC对化合物进行表征和定量。从1公斤干燥的香蕉叶粉中获得124克含5.3％芦丁的粗乙醇提取物。在一系列溶剂分配分离之后，依次使用己烷/二氯甲烷和二氯甲烷/水来自分配的产物的芦丁含量为40.6％。使用Sephadex柱进行纯化可得到级分，其芦丁的纯度最高可达74–84％，然后使用半制备型RP-HPLC进行进一步纯化，可得到最终产物，其芦丁的收率为32.4 mg / g粗提物，纯度为98.4 ％。结果显示香蕉叶（M. balbisiana），食品工业的副产品和农业废料，有潜力用作廉价的芦丁新来源。