The efficacy of a Sabin-derived inactivated polio vaccine (sIPV) can be evaluated by measuring the immunogenicity and the contents of D-antigens, which induce the neutralizing antibodies. The immunogenic potency test in rats was done as a national assay in Japan. The two manufacturers of sIPV in Japan have performed both assays since development, and there is no clear discrepancy between the results obtained in the two assays. To further know the relationship between the two assays, we analyzed the effects of the heat treatment of sIPV on the D-antigenicity and the immunogenicity. We observed that the marginal D-antigen that remained after the thermal treatment was capable of inducing relatively high neutralizing antibodies in rats. This indicates that the measurement of the D-antigen contents as part of the quality control of sIPV is more sensitive and appropriate to detect denatured vaccines.

可以通过测量诱导中和抗体的免疫原性和D抗原含量来评估Sabin衍生的灭活脊髓灰质炎疫苗（sIPV）的功效。大鼠的免疫原性效能测试是日本的一项国家测定。自开发以来，日本的sIPV的两家制造商都进行了两种测定，并且在两种测定中获得的结果之间没有明显的差异。为了进一步了解这两种测定法之间的关系，我们分析了sIPV热处理对D抗原性和免疫原性的影响。我们观察到热处理后残留的边缘D抗原能够在大鼠中诱导相对较高的中和抗体。