Abstract The importance of physiologically realistic in vitro skin models in development of cosmetics and drugs for skin disorders have been well recognized, but current in vitro skin models carry several limitations in recapitulating the physiology of the human skin. Recently, in vitro skin models in a microfluidic platform have been proposed with improved physiological relevance. Survival and differentiation of skin tissue construct requires accurate refinement of cell culture environment, which can be different for static and dynamic culture conditions. In this study, we studied the effect of different culture conditions on the skin construct in a microfluidic skin chip. Our observation suggests that different seeding cell concentrations, thickness of the collagen matrix, volume of cell culture media and the frequency of media exchange affects the cell viability, extent of collagen gel contraction, and adherence of keratinocytes to the gel matrix. Our study provides valuable information about optimization of cell culture conditions in a microfluidic skin chip.

中文翻译：

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培养条件对皮肤切片中细胞活力和凝胶收缩的影响

摘要 生理上逼真的体外皮肤模型在化妆品和皮肤病药物开发中的重要性已得到广泛认可，但目前的体外皮肤模型在概括人类皮肤的生理学方面存在一些局限性。最近，已经提出了具有改进生理相关性的微流体平台中的体外皮肤模型。皮肤组织构建体的存活和分化需要准确细化细胞培养环境，静态和动态培养条件可能不同。在这项研究中，我们研究了不同培养条件对微流体皮肤芯片中皮肤结构的影响。我们的观察表明，不同的接种细胞浓度、胶原基质的厚度、细胞培养基的体积和培养基交换的频率会影响细胞活力、胶原凝胶收缩程度以及角质细胞对凝胶基质的粘附。我们的研究提供了有关优化微流体皮肤芯片中细胞培养条件的宝贵信息。