Recently, the use of atomic spectrometry (AS) for biochemical analysis has attracted considerable attention due to its high sensitivity, selectivity and anti-interference ability. In this work, we conducted a detailed study on a phenomenon of thiol inhibition of mercury (Hg2+) cold vapor generation (CVG) and found L-cysteine (L-Cys), glutathione (GSH), dithiothreitol, N-Acetyl-L-cysteine, 3-mercaptopropionic acid, β-mercaptoethanol, and NaI can inhibit the CVG of Hg2+, while EDTA has no inhibitory effect. Furthermore, changing the content of -SH can effectively adjust the CVG atomic fluorescence spectrometer (CVG-AFS) signal of Hg2+. As as a consequence, an AS-based homogeneous bioassay was constructed by adjusting the oxidation ratio and production quantity of -SH in the system. The quantitative analysis of the system was demonstrated by using AFS as a representative detector. Hydrogen peroxide (H2O2) and glucose were used as representative analytes for the validation of Hg2+ atomic fluorescence signal turn-off strategy, and butyrylcholinesterase (BChE) as well as parathion (organophosphorus pesticides, OPs) as utilized as representative targets for the signal turn-on strategy. Under optimal experimental conditions, the homogeneous CVG-AFS sensor can be successfully used to detect 3 μM H2O2, 30 μM glucose, 0.25 U/L BChE, and 0.4 μg/mL parathion. In addition, the detection results of glucose and BChE in human serum samples agreed well with those obtained by using glucometer and kit, showing the promising potential of this method for practical applications. Therefore, this work provides a perspective for the construction of AS-based homogeneous bioassays and shows great potential for the detection of biomarkers.

中文翻译：

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在 SnCl2/NaBH4 系统中硫醇抑制 Hg 冷蒸汽生成：通过原子光谱法检测 H2O2/葡萄糖和丁酰胆碱酯酶/农药的均相生物测定

近年来，利用原子光谱法（AS）进行生化分析因其高灵敏度、高选择性和抗干扰能力而备受关注。在这项工作中，我们对硫醇抑制汞 (Hg2+) 冷蒸气生成 (CVG) 的现象进行了详细研究，发现了 L-半胱氨酸 (L-Cys)、谷胱甘肽 (GSH)、二硫苏糖醇、N-乙酰-L-半胱氨酸、3-巯基丙酸、β-巯基乙醇、NaI可抑制Hg2+的CVG，而EDTA无抑制作用。此外，改变-SH的含量可以有效调节Hg2+的CVG原子荧光光谱仪（CVG-AFS）信号。因此，通过调整系统中-SH的氧化率和产量，构建了基于AS的均相生物测定。系统的定量分析通过使用 AFS 作为代表性检测器来证明。过氧化氢 (H2O2) 和葡萄糖用作验证 Hg2+ 原子荧光信号关闭策略的代表性分析物，丁酰胆碱酯酶 (BChE) 和对硫磷（有机磷农药，OPs）用作信号转换的代表性目标在战略上。在最佳实验条件下，均质 CVG-AFS 传感器可成功用于检测 3 μM H2O2、30 μM 葡萄糖、0.25 U/L BChE 和 0.4 μg/mL 对硫磷。此外，人血清样品中葡萄糖和BChE的检测结果与使用血糖仪和试剂盒获得的结果吻合良好，显示了该方法在实际应用中的潜力。所以，