Bioselenols are important substances for the maintenance of physiological balance and offer anticancer properties; however, their causal mechanisms and effectiveness have not been assessed. One way to explore their physiological functions is the in vivo detection of bioselenols at the molecular level, and one of the most efficient ways to do so is to use fluorescent probes. Various types of bioselenol-specific fluorescent probes have been synthesized and optimized using chemical simulations and by improving biothiol fluorescent probes. Here, we review recent advances in bioselenol-specific fluorescent probes for selenocysteine (Sec), thioredoxin reductase (TrxR), and hydrogen selenide (H2Se). In particular, the molecular design principles of different types of bioselenols, their corresponding sensing mechanisms, and imaging applications are summarized.

中文翻译：

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生物硒醇特异性荧光探针的综述：合成、特性和成像应用

生物硒醇是维持生理平衡的重要物质，具有抗癌特性；然而，尚未对其因果机制和有效性进行评估。探索其生理功能的一种方法是在分子水平上对生物硒醇进行体内检测，而最有效的方法之一是使用荧光探针。已经使用化学模拟和通过改进生物硫醇荧光探针合成和优化了各种类型的生物硒醇特异性荧光探针。在这里，我们回顾了硒代半胱氨酸 (Sec)、硫氧还蛋白还原酶 (TrxR) 和硒化氢 (H2Se) 的生物硒醇特异性荧光探针的最新进展。特别是不同类型生物硒醇的分子设计原理，它们对应的传感机制，