In the review, the principles and main purposes of using multienzyme systems in electrochemical biosensors are analyzed. Coupling several enzymes allows an extension of the spectrum of detectable substances, an increase in the biosensor sensitivity (in some cases, by several orders of magnitude), and an improvement of the biosensor selectivity, as showed on the examples of amperometric, potentiometric, and conductometric biosensors. The biosensors based on cascade, cyclic and competitive enzyme systems are described alongside principles of function, advantages, disadvantages and practical use for real sample analyses in various application areas (food production and quality control, clinical diagnostics, environmental monitoring). The complications and restrictions regarding the development of multienzyme biosensors are evaluated. The recommendations on the reasonability of elaboration of novel multienzyme biosensors are given.

中文翻译：

---

基于多酶系统的电化学生物传感器：主要群体、优势和局限性——综述

在综述中，分析了在电化学生物传感器中使用多酶系统的原理和主要目的。偶联几种酶可以扩展可检测物质的光谱，提高生物传感器的灵敏度（在某些情况下，提高几个数量级），并提高生物传感器的选择性，如电流、电位和电导生物传感器。描述了基于级联、循环和竞争酶系统的生物传感器以及功能原理、优点、缺点和在各种应用领域（食品生产和质量控制、临床诊断、环境监测）中实际样品分析的实际用途。评估了多酶生物传感器开发的复杂性和限制。