DNA aptamers were selected for their ability to bind specifically and quickly to crystalline hydroxyapatite (Ca10(PO4)6(OH)2; HAP), the primary mineral component of enamel and bone. Aptamers were found to have an enhanced percent of G-nucleotides and a propensity for forming a G-quadruplex secondary structure. One aptamer was studied in comparison to control sequences and was found to bind with high affinity and at high loading capacity, with enhanced binding kinetics, and with specificity for crystalline HAP material over amorphous calcium phosphate (ACP) and β-tricalcium phosphate (TCP). The fluorescently-functionalized aptamer was demonstrated to specifically label HAP in a surface binding experiment and suggests the usefulness of this selected aptamer in biomedical or biotechnology fields where the labeling of specific calcium phosphate materials is required.

中文翻译：

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选定的 DNA 适体作为羟基磷灰石亲和试剂

选择 DNA 适配体是因为它们能够特异性快速地结合结晶羟基磷灰石（Ca10(PO4)6(OH)2；HAP），这是牙釉质和骨骼的主要矿物成分。发现适体具有增加的 G-核苷酸百分比和形成 G-四链体二级结构的倾向。与对照序列相比，研究了一种适体，发现以高亲和力和高负载能力结合，具有增强的结合动力学，并且与无定形磷酸钙 (ACP) 和 β-磷酸三钙 (TCP) 相比，对结晶 HAP 材料具有特异性.