Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses.
Cell Reports ( IF 7.5 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.celrep.2020.02.083 Lydia Maus 1 , ChoongKu Lee 2 , Bekir Altas 3 , Sinem M Sertel 4 , Kirsten Weyand 2 , Silvio O Rizzoli 4 , JeongSeop Rhee 2 , Nils Brose 2 , Cordelia Imig 2 , Benjamin H Cooper 2
Cell Reports ( IF 7.5 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.celrep.2020.02.083 Lydia Maus 1 , ChoongKu Lee 2 , Bekir Altas 3 , Sinem M Sertel 4 , Kirsten Weyand 2 , Silvio O Rizzoli 4 , JeongSeop Rhee 2 , Nils Brose 2 , Cordelia Imig 2 , Benjamin H Cooper 2
Affiliation
|
Although similar in molecular composition, synapses can exhibit strikingly distinct functional transmitter release and plasticity characteristics. To determine whether ultrastructural differences co-define this functional heterogeneity, we combine hippocampal organotypic slice cultures, high-pressure freezing, freeze substitution, and 3D-electron tomography to compare two functionally distinct synapses: hippocampal Schaffer collateral and mossy fiber synapses. We find that mossy fiber synapses, which exhibit a lower release probability and stronger short-term facilitation than Schaffer collateral synapses, harbor lower numbers of docked synaptic vesicles at active zones and a second pool of possibly tethered vesicles in their vicinity. Our data indicate that differences in the ratio of docked versus tethered vesicles at active zones contribute to distinct functional characteristics of synapses.
中文翻译：
海马突触中突触前功能异质性的超微结构相关性。
尽管分子组成相似，但突触可表现出截然不同的功能性递质释放和可塑性特征。为了确定超微结构差异是否共同定义了这种功能异质性，我们将海马器官型切片培养，高压冷冻，冷冻替代和3D电子断层扫描相结合，以比较两个功能不同的突触：海马Schaffer侧支和苔藓纤维突触。我们发现，与Schaffer侧突触相比，苔藓纤维突触具有更低的释放概率和更强的短期促进作用，在活动区和邻近区域的第二个可能系留的突触囊泡容纳的突触囊泡数量较少。
更新日期：2020-03-19
中文翻译：
海马突触中突触前功能异质性的超微结构相关性。
尽管分子组成相似，但突触可表现出截然不同的功能性递质释放和可塑性特征。为了确定超微结构差异是否共同定义了这种功能异质性，我们将海马器官型切片培养，高压冷冻，冷冻替代和3D电子断层扫描相结合，以比较两个功能不同的突触：海马Schaffer侧支和苔藓纤维突触。我们发现，与Schaffer侧突触相比，苔藓纤维突触具有更低的释放概率和更强的短期促进作用，在活动区和邻近区域的第二个可能系留的突触囊泡容纳的突触囊泡数量较少。
京公网安备 11010802027423号