The Borrelia burgdorferi VlsE Lipoprotein Prevents Antibody Binding to an Arthritis-Related Surface Antigen.,Cell Reports

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The Borrelia burgdorferi VlsE Lipoprotein Prevents Antibody Binding to an Arthritis-Related Surface Antigen.
Cell Reports ( IF 7.5 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.celrep.2020.02.081
Abdul G Lone ₁ , Troy Bankhead ₁

Affiliation

Arp is an immunogenic protein of the Lyme disease spirochete Borrelia burgdorferi and contributes to joint inflammation during infection. Despite Arp eliciting a strong humoral response, antibodies fail to clear the infection. Given previous evidence of immune avoidance mediated by the antigenically variable lipoprotein of B. burgdorferi, VlsE, we use passive immunization assays to examine whether VlsE protects the pathogen from anti-Arp antibodies. The results show that spirochetes are only able to successfully infect passively immunized mice when VlsE is expressed. Subsequent immunofluorescence assays reveal that VlsE prevents binding of Arp-specific antibodies, thereby providing an explanation for the failure of Arp antisera to clear the infection. The results also show that the shielding effect of VlsE is not universal for all B. burgdorferi cell-surface antigens. The findings reported here represent a direct demonstration of VlsE-mediated protection of a specific B. burgdorferi surface antigen through a possible epitope-shielding mechanism.

中文翻译：

伯氏疏螺旋体VlsE脂蛋白可防止抗体与关节炎相关的表面抗原结合。

Arp是莱姆病螺旋体疏螺旋体伯氏疏螺旋体的一种免疫原性蛋白，在感染过程中会引起关节发炎。尽管Arp引起强烈的体液反应，但抗体无法清除感染。鉴于以前的证据表明，B。burgdorferi的抗原可变脂蛋白介导了避免免疫的功能，因此我们使用被动免疫试验来检查VlsE是否能保护病原体免受抗Arp抗体的侵害。结果表明，螺旋体仅能在表达VlsE时成功感染被动免疫的小鼠。随后的免疫荧光分析表明，VlsE阻止了Arp特异性抗体的结合，从而为Arp抗血清无法清除感染提供了解释。结果还表明，VlsE的屏蔽作用并非对所有B都通用。burgdorferi细胞表面抗原。此处报道的发现代表通过可能的表位屏蔽机制对VlsE介导的特定伯氏疏螺旋体表面抗原的保护的直接证明。

更新日期：2020-03-19

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