Novel chemosensors based on excited state intramolecular proton transfer chemosensors (F1, F3, F5) and reference dyes (F2, F4, F6) are presented in this work. Target dyes F1, F3 and F5 display high selectivity and sensitivity toward Zn²⁺ in solvents (such as EtOH, EtOH/PBS buffer) in the presence of competitive ions (K+, Na+, Ni2+, Pb2+, Mn2+, Mg2+, Hg2+, Cu2+,Cd2+, Cr3+, Fe3+). Sensing property for Zn²⁺ was studied by UV–Visible, fluorescence spectrophotometric analyses and Job's plot analyses. F1, F3 and F5 for Zn²⁺ detection revealed extremely low detection limit (67.2 nM), high anti-interference ability, and the immediate response. Furthermore, inverted fluorescence microscopy imaging experiment were also investigated, the results demonstrated that the probes have well cell membrane permeability and hypotoxicity. Simultaneously, due to the ability to adapt a wider range of pH, the probes could also image Zn²⁺ in live cells with remarkable fluorescence variation and provide a new method to visualize monitoring in live cells.

在这项工作中提出了基于激发态分子内质子转移化学传感器（F1，F3，F5）和参考染料（F2，F4，F6）的新型化学传感器。目标染料F1，F3和F5显示高选择性和高灵敏度朝向的Zn ²⁺在溶剂（例如EtOH，乙醇/ PBS缓冲液中）在竞争性离子（K +，钠离子，Ni2 +的，对Pb2 +，MN2 +，Mg2 +的和Hg2 +和Cu2 +的存在，Cd2 +，Cr3 +，Fe3 +）。通过UV-可见光，荧光分光光度法分析和Job's图分析研究了Zn ^2+的传感特性。Zn ^2+的F1，F3和F5检测显示极低的检测限（67.2 nM），高抗干扰能力和即时响应。此外，还进行了倒置荧光显微镜成像实验，结果表明该探针具有良好的细胞膜通透性和低毒性。同时，由于能够适应更大范围的pH，这些探针还可以对活细胞中的Zn ²⁺进行成像，并具有显着的荧光变化，并提供了一种可视化活细胞监测的新方法。