Pseudorabies virus (PRV) is an important pathogen that threatens the global swine industry. Currently, there is no effective drug that can clinically prevent or treat PRV infections. Isobavachalcone (IBC), a natural chalcone compound derived from Psoralea corylifolia, displays multiple biological activities, such as antibacterial, antifungal, and anticancer activities. Recently, it was found that IBC exhibited antiviral activity against an RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), in vitro. In the current study, we further demonstrated for the first time that IBC has a strong inhibitory effect on PRV. Through a viral luciferase expression assay, we showed that the inhibition step occurs mainly in the late stage of viral replication. Finally, via a cell-to-cell fusion assay, we demonstrated that IBC inhibits PRV by blocking virus-mediated cell fusion. Thus, IBC may be a candidate for further therapeutic evaluation against PRV infection in vivo.

中文翻译：

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异巴伐康酮通过削弱病毒诱导的细胞间融合来抑制伪狂犬病病毒。

伪狂犬病病毒（PRV）是威胁全球养猪业的重要病原体。当前，没有可以在临床上预防或治疗PRV感染的有效药物。异头孢沙酮（IBC）是衍生自紫叶番荔枝的天然查尔酮化合物，具有多种生物学活性，例如抗菌，抗真菌和抗癌活性。最近，发现IBC在体外对RNA病毒，猪生殖和呼吸综合症病毒（PRRSV）表现出抗病毒活性。在本研究中，我们首次进一步证明了IBC对PRV有很强的抑制作用。通过病毒荧光素酶表达测定，我们表明抑制步骤主要发生在病毒复制的后期。最后，通过细胞间融合测定，我们证明了IBC通过阻断病毒介导的细胞融合来抑制PRV。因此，IBC可能是针对体内PRV感染进行进一步治疗评估的候选药物。