Kidney stone disease (KSD) is more common in individuals with hypertension (HTN) than in individuals with normotension (NTN). Urinary dysbiosis is associated with urinary tract disease and systemic diseases. However, the role of the urinary microbiome in KSD complicated with HTN remains unclear. This study investigated the relationship between the pelvis urinary microbiome and blood pressure (BP) in patients with KSD co-occurring with HTN (KSD-HTN) and healthy controls (HC) by conducting 16S rRNA gene sequencing of bacteria in urine samples. The urine samples were collected (after bladder disinfection) from 50 patients with unilateral kidney calcium stones and NTN (n = 12), prehypertension (pHTN; n = 11), or HTN (n = 27), along with 12 HCs. Principal coordinates analysis showed that there were significant differences in the urinary microbiomes not only between KSD patients and HCs but also between KSD-pHTN or KSD-HTN patients and KSD-NTN patients. Gardnerella dominated in HCs, Staphylococcus dominated in KSD-NTN patients and Sphingomonas dominated in both KSD-pHTN and KSD-HTN patients. The abundance of several genera including Acidovorax, Gardnerella and Lactobacillus was correlated with BP. Adherens junction and nitrogen and nucleotide metabolism pathways, among others, were associated with changes in BP. The findings suggest that patients with KSD complicated with HTN have a unique urinary microbiome profile and that changes in the microbiome may reflect disease progression and may be useful to monitor response to treatments.

中文翻译：

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高血压肾结石患者尿微生物组的特征。

肾结石病（KSD）在高血压（HTN）患者中比在正常血压（NTN）患者中更为常见。泌尿系统疾病与泌尿系统疾病和全身性疾病有关。然而，尿微生物组在KSD合并HTN中的作用尚不清楚。本研究通过对尿液中细菌进行16S rRNA基因测序，研究了HSD并发的KSD患者（KSD-HTN）和健康对照者（HC）的骨盆泌尿微生物组与血压之间的关系。（膀胱消毒后）从50例单侧肾钙结石和NTN（n = 12），高血压前期（pHTN； n = 11）或HTN（n = 27）以及12种HCs的患者中收集尿液样本。主坐标分析表明，不仅KSD患者和HCs之间，而且KSD-pHTN或KSD-HTN患者与KSD-NTN患者之间的尿微生物群存在显着差异。HCs以加德纳菌为主，KSD-NTN患者以葡萄球菌为主，KSD-pHTN和KSD-HTN患者以鞘氨醇单胞菌为主。嗜酸菌属，加德纳菌和乳杆菌等多个属的丰度与血压相关。粘附连接，氮和核苷酸代谢途径等与血压变化有关。研究结果表明，KSD合并HTN的患者具有独特的泌尿微生物组特征，微生物组的变化可能反映疾病的进展，可能有助于监测对治疗的反应。