Hydrolates, complex mixtures containing traces of essential oils (EOs), are inexpensive, easy to make and less toxic than their corresponding EOs. The antibacterial and antifungal activity of the hydrolate of Coridothymus capitatus (L.) Reichenb. fil. (Lamiaceae) alone and in combination with antimicrobial drugs, such as tetracycline and itraconazole, were evaluated. The chemical composition was analysed by gas-chromatography-mass spectrometry (GC-MS). Standard methods were performed to evaluate the susceptibility of some Gram-positive and Gram-negative bacteria, and Candida spp. to the hydrolate, in comparison with its EO. The hydrolate mechanism of action was assayed by propidium iodide and MitoTracker staining. Checkerboard tests were carried out for combinations studies. GC-MS identified 0.14% (v/v) of total EO content into hydrolate and carvacrol as a dominant component. The hydrolate showed a good antimicrobial activity against bacteria and yeasts. It exhibited a synergistic effect with itraconazole against Candida krusei, and an additive effect with tetracycline against methicillin-resistant Staphylococcus aureus strains. Hydrolate changed the membranes permeability of bacteria and yeasts and altered mitochondrial function of yeasts. Our study extends the knowledge by exploiting non-conventional antimicrobial agents to fight the emergence of antibiotic resistance.

中文翻译：

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评价Coridothymus capitatus（L.）Reichenb的水解产物的抗菌活性。菲尔 （唇形科）单独或与抗菌剂组合使用。

含有痕量香精油（EOs）的水合物是复杂的混合物，与相应的EOs相比，价格便宜，易于制造且毒性较低。Coridothymus capitatus（L.）Reichenb水解产物的抗菌和抗真菌活性。fil。（唇形科）单独和与诸如四环素和伊曲康唑的抗微生物药物组合进行了评估。通过气相色谱-质谱法（GC-MS）分析化学成分。进行标准方法以评估某些革兰氏阳性和革兰氏阴性细菌以及假丝酵母菌的敏感性。与它的EO相比 通过碘化丙啶和MitoTracker染色测定了水解的作用机理。进行棋盘测试以进行组合研究。GC-MS识别为0。总EO含量的14％（v / v）以水合物和香芹酚为主要成分。水解物对细菌和酵母菌显示出良好的抗菌活性。它与伊曲康唑对克鲁斯假丝酵母具有协同作用，与四环素对耐甲氧西林的金黄色葡萄球菌菌株具有累加作用。氢氧化物改变了细菌和酵母的膜通透性，并改变了酵母的线粒体功能。我们的研究通过利用非常规抗菌剂来对抗抗生素耐药性的出现，扩展了知识。四环素对耐甲氧西林的金黄色葡萄球菌菌株具有累加作用。氢氧化物改变了细菌和酵母的膜通透性，并改变了酵母的线粒体功能。我们的研究通过利用非常规抗菌剂来对抗抗生素耐药性的出现，从而扩展了知识。四环素对耐甲氧西林的金黄色葡萄球菌菌株具有累加作用。氢氧化物改变了细菌和酵母的膜通透性，并改变了酵母的线粒体功能。我们的研究通过利用非常规抗菌剂来对抗抗生素耐药性的出现，从而扩展了知识。