Alternative splicing (AS) plays a critical regulatory role in modulating transcriptome and proteome diversity. In particular, it increases the functional diversity of proteins. Recent genome-wide analysis of AS using RNA-Seq has revealed that AS is highly pervasive in plants. Furthermore, it has been suggested that most AS events are subject to tissue-specific regulation. To reveal the functional characteristics induced by AS and tissue-specific splicing events, a database for exploring these characteristics is needed, especially in plants. To address these goals, we constructed a database of annotated transcripts generated by alternative splicing in cucumbers (CuAS: http://cmb.bnu.edu.cn/alt_iso/index.php) that integrates genomic annotations, isoform-level functions, isoform-level features, and tissue-specific AS events among multiple tissues. CuAS supports a retrieval system that identifies unique IDs (gene ID, isoform ID, UniProt ID, and gene name), chromosomal positions, and gene families, and a browser for visualization of each gene. We believe that CuAS could be helpful for revealing the novel functional characteristics induced by AS and tissue-specific AS events in cucumbers. CuAS is freely available at http://cmb.bnu.edu.cn/alt_iso/index.php.

中文翻译：

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CuAS：黄瓜中选择性剪接产生的带注释的笔录数据库

选择性剪接（AS）在调节转录组和蛋白质组多样性中起着至关重要的调节作用。特别是，它增加了蛋白质的功能多样性。最近使用RNA-Seq对AS进行全基因组分析表明，AS在植物中普遍存在。此外，已经建议大多数AS事件受到组织特异性调节。为了揭示由AS和组织特异性剪接事件诱导的功能特征，需要用于探索这些特征的数据库，尤其是在植物中。为了实现这些目标，我们构建了一个由黄瓜替代剪接产生的带注释的转录本数据库（CuAS：http://cmb.bnu.edu.cn/alt_iso/index.php），该数据库整合了基因组注释，同工型水平功能，同工型级特征以及多个组织之间的组织特定AS事件。CuAS支持可识别唯一ID（基因ID，同工型ID，UniProt ID和基因名称），染色体位置和基因家族的检索系统，以及用于显示每个基因的浏览器。我们认为，CuAS可能有助于揭示黄瓜中由AS和组织特异性AS事件引起的新型功能特征。CuAS可从http://cmb.bnu.edu.cn/alt_iso/index.php免费获得。