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Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-03-23 , DOI: 10.1021/acs.jafc.0c00297
Justyna Chudzik-Kozłowska 1 , Ewa Wasilewska 1 , Dagmara Złotkowska 1
Affiliation  

Green pea (Pisum sativum) is a component of European cuisine; however, an estimated 0.8% of Europeans suffer from allergies to pea proteins. We examined the immunoreactive potential of pea albumins (PA) in BALB/c and C57BL/6 mice. Mice were orally gavaged with PA or glycated pea albumins (G-PA) for 10 consecutive days, in combination with an adjuvant. Both PA and G-PA increased PA-specific serum antibody titers to about 212 for anti-PA IgG, ∼27 for anti-PA IgA, and ∼27.8 for anti-PA IgA in fecal extracts (p < 0.001). On day 42 postexposure, the antibodies titers decreased and were greater in BALB/c compared to C57BL/6 mice (p < 0.05). Distribution of CD4+ and CD8+ T cells in lymphoid tissues presented strain-specific differences. PA was found to induce lymphocyte proliferation; however, G-PA did not. Both PA and G-PA changed CD4+ and CD8+ T cells percentages in some lymphoid tissues; however, this did not impact cytokines production by splenocyte cultures evidenced by the stimulation of Th1, Th2, and Th17 cells. The observed immunomodulatory properties of PA and G-PA and lack of a sign of allergic reaction render them suitable for supplements in personalized diets, but further research is needed to precisely understand this activity.

中文翻译:


BALB/c 和 C57BL/6 小鼠中豌豆 (Pisum sativum) 白蛋白免疫反应性的评估



青豌豆( Pisum sativum )是欧洲美食的组成部分;然而,估计有 0.8% 的欧洲人对豌豆蛋白过敏。我们检查了 BALB/c 和 C57BL/6 小鼠中豌豆白蛋白 (PA) 的免疫反应潜力。连续 10 天给小鼠口服 PA 或糖化豌豆白蛋白 (G-PA),并结合佐剂。 PA 和 G-PA 均将粪便提取物中抗 PA IgG 的 PA 特异性血清抗体滴度增加至约 2 12 ,抗 PA IgA 增加至约 2 7 ,抗 PA IgA 增加至约 2 7.8p < 0.001)。暴露后第 42 天,与 C57BL/6 小鼠相比,BALB/c 小鼠的抗体滴度下降且更高 ( p < 0.05)。淋巴组织中CD4 +和CD8 + T细胞的分布呈现出菌株特异性差异。 PA 被发现可诱导淋巴细胞增殖;然而,G-PA 却没有。 PA和G-PA都改变了一些淋巴组织中CD4 +和CD8 + T细胞的百分比;然而,这并不影响脾细胞培养物产生的细胞因子,这由 Th1、Th2 和 Th17 细胞的刺激所证明。观察到的 PA 和 G-PA 的免疫调节特性以及缺乏过敏反应的迹象使它们适合个性化饮食中的补充剂,但需要进一步的研究来准确了解这种活性。
更新日期:2020-03-24
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